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Table 2 The intermediate precision and accuracy of the developed RT-qPCR infectivity assay is determined

From: Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approach

Assay # RT-qPCR (pfu) RT-qPCR (log_pfu) Plaque assay Accuracy% CV%
(Mean from 30 assays)
1 1.50E + 07 16.52 1.41E + 07 106.38  
2 1.63E + 07 16.60 115.60
3 1.45E + 07 16.48 102.34
4 1.70E + 07 16.64 120.57
5 1.54E + 07 16.54 109.22
6 1.31E + 07 16.38   92.91 9.19
  1. RT-qPCR infectivity assay was performed six times by two analysts on different days. The accuracy of the assay was calculated by evaluating the percentages of values obtained by RT-qPCR infectivity assay versus the expected infectious titre values by plaque assay. The CV% from the six independent assays is also determined.