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Table 1 Infectious titre results obtained by RT-qPCR infectivity assay or plaque assay

From: Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approach

A.

 

0-1 hr

1 day

3 days

6 days

7 days

RT-qPCR infectivity

7.50E + 06

7.28E + 06

4.35E + 06

3.35E + 06

2.43E + 06

Plaque assay

7.36E + 06

5.55E + 06

4.52E + 06

4.43E + 06

2.70E + 06

B.

RT-qPCR infectivity

3.06E + 06

1.14E + 06

2.14E + 06

1.30E + 06

3.78E + 05

Plaque assay

3.23E + 06

3.40E + 06

2.80E + 06

1.55E + 06

N/A

  1. HSV529 test samples were incubated at A. 4–8°C or B. 22-25°C at various time points and the infectious titre was measured by RT-qPCR infectivity assay or plaque assay.