Figure 3From: Evaluation of infectious titer in a candidate HSV type 2 vaccine by a quantitative molecular approachOverview of the developed RT-qPCR infectivity assay. Ninety six well plates were pre-seeded with AV529-19. Next day, cells were infected with the serial dilutions of HSV529 or in-house reference control. RNA was extracted 16Â hours post infection, treated with DNase, RT-qPCR performed targeting HSV-2 gD2, and infectious titer assigned by PLA analysis.Back to article page