Identification of new developmentally regulated genes involved in Streptomyces coelicolorsporulation

BMC Microbiology

Table 1 Fluorescence-based assays of promoter activity

Average fluorescence intensity (arbitrary unit)
	Spores		Vegetative hyphae
Strain	Avg^a	95CI	Avg^a	95CI^e
M145	19.0	16.2 - 21.9	3.51	-5.73 - 12.8
pKF210	21.3^c	17.8 - 24.8	-11.1	-23.1 - 0.940
SCO0934^b	68.7^d	65.3 - 72.1	-18.7	-26.9 - -10.4
SCO1773^b	35.5^d	32.2 - 38.9	18.1	2.20 - 34.0
SCO1774^b	1467^d	1440 - 1493	14.3	1.39 - 27.2
SCO3857^b	1077^d	1048 - 1105	6.08	-2.98 - 15.1
SCO4157^b	93.4^d	90.1 - 96.7	12.33	4.39 - 20.3
SCO4421^b	586^d	568 - 604	6.02	2.04 - 10.0
SCO7449^b	831^d	805 - 856	15.7	8.87 - 22.5

^aAverage intensity value per pixel after subtraction of background signals from the medium. The fluorescence intensity was measured in areas of 0.22 μm² per spore (totally between 454–743 spores per strain) and in 50 randomly selected areas (0.22 μm²) of the surrounding medium.
^bPromoter region of corresponding gene translationally fused to the gene encoding the fluorescent protein mCherry (mCh) in pKF210, integrated into the chromosome of M145.
^cDifference from M145 not significant (P = 0.37) according to Student’s t test.
^dDifference from M145/pKF210 highly significant (P > 0.001) according to Student’s t test.
^e95% confidence interval.

ISSN: 1471-2180