Figure 5From: Identification of new developmentally regulated genes involved in Streptomyces coelicolorsporulationQuantitative real-time RT-PCR assays of selected genes. Specific primer pairs were used to amplify SCO0934, SCO1195, SCO1773, SCO1774, SCO3857, SCO7449 , and hrdB from cDNA prepared from cultures of the parent M145 (marked with W), J2401 (whiA mutant, marked with A) and J2408 (whiH mutant, marked with H) after 18Â h, 36Â h and 48Â h of growth. The assay for each gene was calibrated to the absolute concentration of template per ml reaction volume. Error bars show standard deviations from a total of six assays.Back to article page