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Table 5 Composition of oral Treponema isolation (OTI) and basal minimal media with VFA (BMV) media used in these studies

From: Biochemical and molecular characterization of Treponema phagedenis-like spirochetes isolated from a bovine digital dermatitis lesion

Component

OTI

BMV

Polypeptone

5.0 g

5.0 g

Heart Infusion Broth

5.0 g

5.0 g

Yeast Extract (YE)

5.0 g

1.0 g

Glucose

0.8 g

†

Pectin

0.8 g

†

Soluble Starch

0.8 g

†

Arabinose

 

†

Casein Digest

 

†

Cellobiose

 

†

Fructose

 

†

Mannitol

 

†

Galactose

 

†

Lactose

 

†

Trehalose

 

†

Mannose

 

†

Sucrose

0.8 g

†

Maltose

0.8 g

†

Ribose

0.8 g

†

Xylose

0.8 g

†

Sodium Pyruvate

0.8 g

†

K2HPO4

2.0 g

2.0 g

NaCl

5.0 g

5.0 g

MgSO4

0.1 g

0.1 g

Cysteine-HCl

0.68 g

1.0 g

DI Water

500 ml

822 ml

Resazurin (0.1%)

1.0 ml

1.0 ml

Rumen Fluid

500 ml

 

VFA Solution**

 

10 ml

Bovine Serum§

1 ml/10 ml

1 ml/10 ml

  1. † - To test carbohydrate substrates 0.5 ml of a 10% solution of each was added to 8.5 ml media just before reduction and inoculation.
  2. **VFA Solution consisted of 0.5 ml each of isovaleric, isobutyric, n-valeric, and DL-a-methylbutyric acid in 100 ml 0.1 N NaOH.
  3. § Final concentration = 10% Bovine serum, added to 8.5 ml medium just before reduction and inoculation.
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BMC Microbiology

ISSN: 1471-2180

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