Table 1 Primers targeting some metal-resistance genes used in this study
Primer name | Mechanism involved/metal involved | Sequence forward (5’-3’) | Sequence reverse (5’-3’) | Annealing temperature | Amplicon size (bp) |
|---|---|---|---|---|---|
copA | Sequestration and transport/Cu | TCCATACACTGGCACGGCAT | TGGATCGGGTGAGTCATCAT | 54 | 1331 |
copB | Sequestration and transport/Cu | TCCACGTTTGTTCACTGCTC | AGTCGGCTGTATTGCCGTAG | 53 | 900 |
copC | Sequestration and transport/Cu | TGTTGAACCGCACAAGTTTC | GGTAATCGGGTGGGTATCG | 54 | 350 |
cnrC2 | RND (Efflux)/Co and Ni | GAGGAAGCGCTGGATTCC | GCAATTCCATCAAAGTTGTCTTGCC | 55 | 341 |
cnrA3 | RND (Efflux)/Co and Ni | GGACATTACCAACAAGCAGG | CACAAACGTCAGCGACAG | 51.5 | 1447 |
chrB | CHR transporter (efflux/reduction)/Cr | GTCGTTAGCTTGCCAACATC | CGGAAAGCAAGATGTCGATCG | 57 | 450 |
czcD | Cation diffusion facilitator (efflux)/Co, Zn and Cd | TTTAGATCTTTTACCACCATGGG CGCAGGTCACTCACACGACC | TTTCAGCTGAACATCATACCCTAGTT TCCTCTGCAGCAAGCGACTTC | 57 | 1000 |
nccA | RND (Efflux)/Ni, Co, Cd | ACGCCGGACATCACGAACAAG | CCAGCGCACCGAGACTCATCA | 57 | 1141 |