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Table 2 Evaluation of purification procedures and their modifications by fluorescence microscopy

From: Development of a flow-fluorescence in situhybridization protocol for the analysis of microbial communities in anaerobic fermentation liquor

Procedure Cell aggregates present Maximum cell aggregate size1) Abiotic particles present Abiotic particles covered with cells
1-C1-S1-H1-F1 yes +++ yes no
1-C1-S1-H2-F1 yes ++ yes no
1-C2-S1-H1-F1 yes ++ yes no
1-C2-S1-H2-F1 yes + yes no
1-C2-S2-H1-F1 no - yes no
1-C2-S2-H1-F2 no - no no
2-C1-S1-H1 yes +++ yes yes
2-C1-S1-H2 yes +++ yes yes
3-C1-S1-H1 yes +++ yes yes
3-C1-S1-H2 yes ++ yes yes
3-C1-S2-H1 yes ++ yes yes
3-C1-S2-H2 yes + yes yes
3-C2-S1-H1 yes +++ yes yes
3-C2-S1-H2 yes ++ yes yes
3-C2-S2-H1 yes ++ yes yes
3-C2-S2-H2 yes ++ yes yes
3-C3-S1-H1 yes ++ yes yes
3C3-S1-H2 yes ++ yes yes
3-C3-S2-H1 yes ++ yes yes
3-C3-S2-H2 yes + yes yes
4-C1-H1 yes +++ yes yes
5-C1-S1-H1 yes +++ yes yes
5-C1-S2-H1 yes +++ yes yes
5-C1-S1-H2 yes ++ yes yes
5-C1-S2-H2 yes ++ yes yes
5-C2-S1-H1 yes +++ yes yes
5-C2-S2-H1 yes +++ yes yes
5-C2-S1-H2 yes ++ yes yes
5-C2-S2-H2 yes + yes yes
6-C1-S1-H1 yes ++ yes yes
  1. 1) +++ = ≥ 52 μm2; ++ = ≥ 24 μm2; + = ≥ 6 μm2; - = no cell aggregates. The size of cell aggregates was determined by microscopic field analyses using an ocular micrometer at 630× magnification. One field covered an area of 5.76 μm2.
  2. Denomination of procedures is according to Table 1. The optimal combination is given in italics.