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Table 1 Purification procedures and modifications

From: Development of a flow-fluorescence in situhybridization protocol for the analysis of microbial communities in anaerobic fermentation liquor

Procedures References Detergents Detergent concentrations (C) Ultrasound treatment (S)1) Homogenization (H)2) Filtration (F)
1 S.B. Singh-Verma (1968), LR. Bakken (1985) Sodium hexametaphosphate C1) 0,2% (w/v) S1) 40 W, 60 sec, 5 impulses/sec (different repetitions) H1) none F1 none
   C2) 0,5% (w/v) S2) 65 W, 60 sec, 5 impulses/sec (different repetitions) H2) 60 sec, speed 5 (different repetitions) F2) 12–15 μm filter
2 S.B. Singh-Verma (1968), LR. Bakken (1985) Bromhexine hydrochloride C1) 0,2% (w/v) S1) 40 W, 60 sec + 65 W, 60 sec, 5 impulses/sec H1) none n.a.
     H2) 2× 60 sec, speed 5  
3 W.B. Yoon and R.A. Rosson (1990) Tween C1) 5 μg/ml S1) 15 W, 30 sec, 5 impulses/sec H1) none n.a.
   C2) 10 μg/ml S2) 35 W, 30 sec, 5 impulses/sec H2) 5 min, speed 5  
   C3) 25 μg/ml    
4 E.L Schmidt (1974) Tween 80 + 0.007 g ml-1 flocculation reagent (Ca (OH)2: MgCO3 (2:5)) C1) 25 μl/ml n.a. n.a. n.a.
5 O. Resina-Pelfort et al. (2003) Triton X-100 C1) 10 μg/ml S1) 35 W, 30 sec, 5 impulses/sec H1) none n.a.
   C2) 20 μg/ml S2) 45 W, 30 sec, 5 impulses/sec H2) 5 min, speed 5  
6 L R. Bakken (1985) Sodium pyrophosphate C1) 0,2% (w/v) S1 3× 40 W, 60 sec, 5 impulses/sec H1) 3× 60 sec, speed 5 n.a.
  1. n.a. = not applied.
  2. 1)using the Sonoplus GW2070 (Bandelin, Germany).
  3. 2)using the dispersion unit VDI12 for 0.1 - 5.0 ml volumes (VWR, Germany).
  4. C1-3, H1-2, S1-2 and F1-2 indicate variations of the original protocols tested for their eligibility on samples from pure cultures and the UASS biogas reactor.