Skip to main content
Figure 4 | BMC Microbiology

Figure 4

From: Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes

Figure 4

Identification of L. acidophilus (La) in a mixture of bacteria extracted from yogurt. A) La was identified in different bacterial extractions only when the α-La1 scFv is used in the staining. Single or multiple cells were sorted using pre-sort (P1), negatively sorted (P2) and positively sorted (P3) gates. B) 16 s rRNA sequencing of single cells sorted from all three gates revealed significant enrichment of L. acidophilus from an average of 3.4% (95% CI: 2.1-4.8%) in the pre-sort (P1) community to 90.6% (95% CI: 86.6-94.6%) in P3 (n = 4, p-value <2.2x10-16 when using a standard Chi-squared test).

Back to article page