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Figure 2 | BMC Microbiology

Figure 2

From: Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes

Figure 2

The antigen recognized by the α-La scFv is the S-layer protein A. A) Western blot using α-La scFv as primary antibody and α-SV5-Alkaline Phosphatase as secondary for detection. An obvious ~45KDa band appeared in the lane containing L. acidophilus (La) lysate and not the lane containing L. johnsonii (Lj) lysate was extracted and identified using MS/MS. B) Protein alignment of S-layer proteins from closely related Lactobacillus species (La = Lactobacillus acidophilus, Lh = Lactobacillus helveticus, Lo = Lactobacillus oris). The two La peptide sequences recovered after MS/MS analysis are indicated with solid triangles or circles above the sequence.

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BMC Microbiology

ISSN: 1471-2180

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