Figure 5

Transcriptional terminators within the B. mycoides dcw and spoIIG gene clusters. Red labels mark the position of the putative terminators. The DX termination sequences displayed are 100% identical to those predicted at the TransTerm-HP site for B. weihenstephanensis KBAB4 (Accession NC_010184, from coordinates 3780796 to 3790953). The green label between ftsA and ftsZ indicates a hairpin structure not recognized there as a potential terminator. The three large green bars over the genes represent the main ftsZ-specific RNAs and the green thin bars the minor ones. The primers used to detect RNA 5’ ends by primer extension are indicated below the genes. The curved arrows in the enlarged region show the main ftsA and ftsZ RNA start sites.