Figure 2From: Transcriptional analysis of ftsZ within the dcw cluster in Bacillus mycoidesDetermination of ftsZ, ftsA and ftsQ RNA 5’ ends by primer extension (PE) in B. mycoides SIN (S) and DX (D). 5’ 32P-labeled primers were hybridized to total RNA, extended by reverse transcriptase and the cDNAs separated by 6% urea-PAGE electrophoresis. The numbers on the right side of the autoradiograms indicate the position of the cDNA 3’ ends relative to the ORF first nucleotide (+1). The thick lateral bar indicates the approximate position in the gel of the next upstream gene. A) PE from primer ZB annealing to ftsZ RNA at +103; B) PE from primer Arev annealing to ftsA RNA at +80; C) PE from primer Qrev annealing to ftsQ RNA at +52 (Table 1). Here two different SIN cDNA preparations were loaded on the gel. A schematic view of the major cDNA products is shown in the inset. M = MW marker 32P-labeled DNAs. GATC = 35S-dATP labeled M13mp18 ladder.Back to article page