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Table 2 Relative abundance of RNase III-dependent or -independent transcripts by different level of YmdB or RNase III

From: Escherichia coliYmdB regulates biofilm formation independently of its role as an RNase III modulator

RNase III-dependent genes

Microarray1

qPCR-Δ ymdB2

qPCR-YmdB3

qPCR-rnc144

mltD

3.66

3.06 ± 0.04

7.37 ± 0.03

39.80 ± 0.01

PnP

3.06

0.84 ± 0.01

3.27 ± 0.36

8.02 ± 0.02

plsX

3.01

2.98 ± 0.01

2.86 ± 0.31

21.37 ± 0.01

ahpF

2.48

0.90 ± 0.02

3.34 ± 0.33

7.72 ± 0.01

yhdE

2.26

1.90 ± 0.01

2.37 ± 0.20

3.93 ± 0.01

RNase III-independent genes

Microarray 1

qPCR- Δ ymdB 2

qPCR-YmdB 3

qPCR- rnc14 4

pspB

5.18

0.88 ± 0.13

1.53 ± 0.01

1.36 ± 0.01

pspA

4.46

0.78 ± 0.01

1.50 ± 0.01

1.15 ± 0.01

pspD

4.30

0.82 ± 0.01

2.45 ± 0.06

1.86 ± 0.02

pspC

3.86

1.01 ± 0.01

1.59 ± 0.02

1.38 ± 0.02

ahpC

2.81

0.67 ± 0.01

3.73 ± 0.01

3.30 ± 0.01

  1. 1Fold-change of each transcript levels from microarray analysis (Additional file 1: Table S3): YmdB overexpression from ASKA-ymdB(−) vs pCA24N (−gfp) in wild-type (BW25113) background.
  2. Relative ratios of each transcript levels determined by qPCR with specific primers (Additional file 1: Table S2) are indicated: 2ymdB knockout (ΔymdB: KSK002) vs BW25113 (ymdB), 3YmdB overexpression from ASKA-ymdB (−) vs pCA24N (−gfp) or 4RNase III mutant (rnc14:KSK001) vs BW25113 (rnc+).