DH5α donor strain | Selection | Transfer frequencya | No. transconjugantsc | No. pA/C positived | No. pX1 positivee |
---|
pX1ydgA::Tn5 | Km | 10-1 | 8 | 0 | 8 |
pX1taxB::Km | Km | 0b | 0 | 0 | 0 |
pA/C, pX1ydgA::Tn5 | Km | 1 to 10-2 | 4 | 0 | 4 |
| CRO | 10-5 to 10-7 | 6 | 6 | 6 |
| CRO-Km | 10-5 to 10-7 | 12 | 12 | 12 |
pA/C, pX1taxB::Km | Km | 0 | 0 | 0 | 0 |
| CRO | 0 | 0 | 0 | 0 |
| CRO-Km | 0 | 0 | 0 | 0 |
- aThe frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.
- bNo transconjugants were detected under the detection level (<10-10).
- cNumber of transconjugants analyzed.
- dNumber of transconjugants positive for the repA/C PCR marker.
- eNumber of transconjugants positive for the oriX1 PCR marker.