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Table 5 Conjugation experiments for pA/C and pX1 mutants using DH5α as recipient

From: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

DH5α donor strain

Selection

Transfer frequencya

No. transconjugantsc

No. pA/C positived

No. pX1 positivee

pX1ydgA::Tn5

Km

10-1

8

0

8

pX1taxB::Km

Km

0b

0

0

0

pA/C, pX1ydgA::Tn5

Km

1 to 10-2

4

0

4

 

CRO

10-5 to 10-7

6

6

6

 

CRO-Km

10-5 to 10-7

12

12

12

pA/C, pX1taxB::Km

Km

0

0

0

0

 

CRO

0

0

0

0

 

CRO-Km

0

0

0

0

  1. aThe frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.
  2. bNo transconjugants were detected under the detection level (<10-10).
  3. cNumber of transconjugants analyzed.
  4. dNumber of transconjugants positive for the repA/C PCR marker.
  5. eNumber of transconjugants positive for the oriX1 PCR marker.