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Table 5 Conjugation experiments for pA/C and pX1 mutants using DH5α as recipient

From: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

DH5α donor strain Selection Transfer frequencya No. transconjugantsc No. pA/C positived No. pX1 positivee
pX1ydgA::Tn5 Km 10-1 8 0 8
pX1taxB::Km Km 0b 0 0 0
pA/C, pX1ydgA::Tn5 Km 1 to 10-2 4 0 4
  CRO 10-5 to 10-7 6 6 6
  CRO-Km 10-5 to 10-7 12 12 12
pA/C, pX1taxB::Km Km 0 0 0 0
  CRO 0 0 0 0
  CRO-Km 0 0 0 0
  1. aThe frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.
  2. bNo transconjugants were detected under the detection level (<10-10).
  3. cNumber of transconjugants analyzed.
  4. dNumber of transconjugants positive for the repA/C PCR marker.
  5. eNumber of transconjugants positive for the oriX1 PCR marker.