BMC Microbiology

Table 4 Description of the pA/C and pA/C + pX1 transconjugants obtained from the YU39 donor

From: Conjugative transfer of an IncA/C plasmid-borne bla_CMY-2gene through genetic re-arrangements with an IncX1 plasmid

		pX1 PCR typing						Second round conjugation^a
Recipient	pA/C colony	oriX1	ydgA	taxB	taxC	ddp3	046-047	Original	DH5α
SO1	IA4	-	-	-	-	-	-	0^b	0
	IA5	-	-	-	-	-	-	0	0
	IA9	-	-	-	-	-	-	0	0
	IIIA4	+	+	+	+	+	+	10^-8	1 to 10^-2
DH5α	221-1	+	+	+	+	+	+	10^-1 to 10^-3
	221-10	+	+	+	+	+	+	1 to 10^-2
	225-1	+	+	+	+	+	+	1 to 10^-2
	225-7	+	+	+	+	+	+	10^-1 to 10^-2
HB101	IIIC9	+	+	+	+	+	+	10^-2 to 10^-4	1 to 10^-2
	IVC8	+	+	+	+	+	+	10^-2	10^-1 to 10^-2
HB101	IIID8	+	+	-	-	-	-	10^-5	10^-5 to 10^-7
(pSTV::Km)	IVD2	+	+	+	+	+	+	1 to 10^-2	0
LT2 (pSTV::Km)	IIIE9	-	-	-	-	-	-	0	10^-4 to 10^-7

^aTransconjugants were challenged for a second round of conjugation using as recipients the original recipient strain (original) or DH5α. The frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.
^bNo transconjugants were detected under the detection level (<10^-10).

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