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Table 4 Description of the pA/C and pA/C + pX1 transconjugants obtained from the YU39 donor

From: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

  pX1 PCR typing Second round conjugationa
Recipient pA/C colony oriX1 ydgA taxB taxC ddp3 046-047 Original DH5α
SO1 IA4 - - - - - - 0b 0
  IA5 - - - - - - 0 0
  IA9 - - - - - - 0 0
  IIIA4 + + + + + + 10-8 1 to 10-2
DH5α 221-1 + + + + + + 10-1 to 10-3  
  221-10 + + + + + + 1 to 10-2  
  225-1 + + + + + + 1 to 10-2  
  225-7 + + + + + + 10-1 to 10-2  
HB101 IIIC9 + + + + + + 10-2 to 10-4 1 to 10-2
  IVC8 + + + + + + 10-2 10-1 to 10-2
HB101 IIID8 + + - - - - 10-5 10-5 to 10-7
(pSTV::Km) IVD2 + + + + + + 1 to 10-2 0
LT2 (pSTV::Km) IIIE9 - - - - - - 0 10-4 to 10-7
  1. aTransconjugants were challenged for a second round of conjugation using as recipients the original recipient strain (original) or DH5α. The frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.
  2. bNo transconjugants were detected under the detection level (<10-10).