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Table 2 First round conjugations for YU39 donor strain

From: Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid

Recipient strain

Transfer frequencya

No. transconjugantsb

No. pA/C positivec

No. pX1 positived

No. ColE1e(% of total)

Typhimurium SO1 (pSTV::Km)

10-8 to 10-10

34

34

1

27 (79)

Typhimurium LT2 (pSTV::Km)

10-8 to 10-10

21

2

19

1 (0.4)

E. coli DH5α

10-7 to 10-9

10

10

10

5 (50)

E. coli HB101

10-7 to 10-8

28

9

21

4 (14)

E. coli HB101 (pSTV::Km)

10-8

28

8

24

4 (14)

  1. aThe frequency was calculated as number of transconjugants per donor; the range in the orders of magnitude obtained is shown.
  2. bNumber of transconjugants analyzed.
  3. cNumber of transconjugants positive for the repA/C PCR marker.
  4. dNumber of transconjugants positive for the oriX1 PCR marker.
  5. eNumber of transconjugants carrying pColE1-like.