LPS induced autophagy is dependent on TLR4 in HMrSV5 cells. (A) Western blot analysis of TLR4, Beclin-1 and LC3-II in HMrSV5 cells treated with LPS at different concentrations for 12 hours or 1 μg/ml LPS for the indicated time periods. β-actin was used as a loading control. (B) Densitometric analysis of the blots showing the ratios of TLR4 to β-actin in Figure 9A. (C) HMrSV5 cells were stimulated for 12 hours in the absence (control) or presence of LPS (1.0 μg/ml), PMB control (100 μg/ml), LPS + PMB. The panel show western blot probed with antibodies against TLR4, Beclin-1, LC3-II or β-action. (D and E) Densitometric analysis of TLR4, Beclin-1 or LC3-II in Figure 9C; β-actin was used as a loading control. Data are mean values ± SD (n ≥3). * and ** denote p < 0.05 and p < 0.01 respectively (vs. control). # and ## denote p < 0.05 and p < 0.01 respectively (vs. LPS).