Figure 6
From: c-KIT signaling is targeted by pathogenic Yersiniato suppress the host immune response
Yersinia infection activates c-KIT tyrosine phosphorylation. THP-1 cells were stimulated with (A) SCF (50 ng/ml) or infected with (B) Y. enterocolitica pYV+ or (C) Y. enterocolitica pYV- at MOI 40 for the indicated time points. Cell lysates were immunoprecipitated with anti-c-KIT antibody followed by Protein A Sepharose and were resolved by 8% SDS-PAGE. Western blots were probed with anti-c-KIT and p-Tyr (PY20) antibodies. Results from three independent experiments were quantified and are presented as percentage of phosphorylated versus total c-KIT.