C. albicansgrowth, transition, biofilm formation, and gene expression modulation by antimicrobial decapeptide KSL-W

Table 4 Gene expression (3 h) under hyphae inducing culture conditions (medium supplemented with 10% fetal calf serum, with culture incubation at 37ºC)

Gene	Untreated *C. albicans*	Amphotericin B		KSL-W 25 μg/ml		KSL-W 100 μg/ml
Gene	Fold change¹	Fold change¹	p-value²	Fold change¹	p-value²	Fold change¹	p-value²
SAP2	0.99	3.36	0.003	0.78	0.02	0.62	0.003
SAP4	0.96	2.41	0.02	0.44	0.0002	0.24	< 0.0001
SAP5	1.00	0.49	0.0007	0.83	0.03	0.01	< 0.0001
SAP6	1.00	2.56	0.01	0.30	< 0.0001	0.11	< 0.0001
EAP1	1.00	6.06	< 0.001	1.06	0.4	0.99	0.8
EFG1	1.00	1.09	0.6	0.55	0.0004	0.66	0.02
NRG1	1.00	2.45	0.01	0.66	0.0006	0.64	0.0005
HWP1	1.00	0.0055	< 0.001	0.078	< 0.0001	0.0035	< 0.0001

¹Fold change was calculated by PCR product of the gene of interest/the PCR product of ACT1 (the house keeping gene), and normalized to the negative control of untreated C. albicans where the expression was considered equal to 1.
²P-values were obtained after comparison of test to negative control (untreated C. albicans).

ISSN: 1471-2180