Table 2 Primers used in this study
Primers for PCR and sequencing | ||
|---|---|---|
Primer name | Primer sequence (5′ → 3′) | Target gene |
IMP F | GCATTGCTACCGCAGCAGAG | blaIMP alleles |
IMP R | GGTTTAAYAAAACAACCAC | |
VIM F | TCGGAGAGGTCCGACTTTACC | blaVIM alleles |
VIM R | CCATTCAGCCAGATCGG | |
SPM-1 F | ACGTTTTCGTCGTCACAG | blaSPM-1 gene |
SPM-1 R | GTCCAGGTATAACAATTTTCG | |
NDM-1 F | AATGGAATTGCCCAATATTATGC | blaNDM-1 gene |
NDM-1 R | TCAGCGCAGCTTGTCGGC | |
OXA-23 F | GATGTGTCATAGTATTCGTCG | blaOXA-23 gene |
OXA-23 R | TCACAACAACTAAAAGCACTG | |
OXA SET A Fa | ATGAAAAAATTTATACTTCC | blaOXA-24-like alleles |
OXA SET A R | TTAAATGATTCCAAGATTTTC | |
OXA SET B F | ACAGAARTATTTAAGTGGG | blaOXA-51-like and blaOXA-58-like alleles |
OXA SET B Ra | GGTCTACAKCCMWTCCCCA | |
OXA SET C F | ATGAAAACATTTGCCGCATA | blaOXA-10-like alleles |
OXA SET C R | CGTTGTCTATATCCATGTTA | |
ISABA1 F | GTGCTTTGCGCTCATCATGC | ISAba1 |
ISABA1 R | CATGTAAACCAATGCTCACC | |
ISABA1 F2b | AGTTGCACTTGGTCGAATGA | Detection of ISAba1 upstream blaOXA-51 and blaOXA-23 |
CARO F | GCTCACCTGATGCTGACATT | carO gene and its promoter |
CARO R | TTGCTTCTTCAACAGCTTGG | |
CARO F SQ | GCGGCTTACCTTGATAACG | carO gene sequencing |
CARO R SQ | TTACGAGCTTCCGCATTTAC | |
Primers for relative quantification | ||
TR CARO F | AGCTTTACTTGCTGCTGGTG | A. baumannii carO transcription |
TR CARO R | CGAGCGCCTACTGGAATTA | |
TR CPN60 F | TTGACCGTGGTTATATCTCTCC | A. baumannii cpn60 transcription |
TR CPN60 R | CGGATTTTCAAGTTCAGCAG | |