Carbapenem-resistant Acinetobacter baumannii from Brazil: role of carO alleles expression and blaOXA-23 gene

Table 1 Genetic and phenotypic features of A. baumannii Brazilian strains analyzed in this study

Strains (n)	PFGE	RQ of carO	CarO classification	bla ^a _OXA	ISAba1	ISAba1+bla_OXA^b	Hodge test	Resistance phenotype	MIC of Imipenem (μg/mL)
AC2-RJ (1)	B	0.41	CarOa	bla _OXA-23	+	+	+	ATM CAZ CIP MEM IPM EPM TMP GEN AMK STR	≥ 32
AC1-RJ (1)	A	1.33	CarOa	bla _OXA-13	+	-	-	ATM CAZ FEP CIP MEM TMP STR	1.0
AC3-RJ (3)	C	1.21	CarOa	bla _OXA-66	+	-	-	ATM CAZ FEP CIP MEM TMP AMK STR	1.0
AC10-RJ (1)	F	1.23	CarOa	bla _OXA-10	+	-	-	ATM CIP MEM TMP GEN AMK STR	1.0
AC6-RJ (3)	D	1.07	Undefined CarO	bla _OXA-13	+	-	-	ATM CAZ FEP CIP MEM IPM EPM TZP TMP GEN AMK STR	32
AC9-RJ (1)	E	0.10	CarOb	bla _OXA-10	+	-	-	ATM CIP MEM TMP GEN STR	0.25
AC16-RJ (2)	G	0.86	CarOb	bla _OXA-10	+	-	-	ATM CAZ FEP CIP MEM TZP TMP AMK STR	1.5
AC72-RJ (1)	H	0.94	CarOa	bla _OXA-23	+	+	+	ATM CAZ CIP MEM IPM EPM TMP AMK STR	≥ 32
AC60-MA (1)	L	0.05	Disrupted by ISAba1	-	+	-	-	MEM EPM	0.20
AC62-MA (2)	J	0.45	CarOa	bla _OXA-23	+	+	+	ATM CAZ FEP CIP MEM IPM EPM TZP TMP AMK STR	≥ 32
AC65-MA (1)	M	0.50	CarOb	bla _OXA-23	+	+	+	ATM CAZ FEP CIP MEM IPM EPM TZP TMP GEN AMK STR	≥ 32
AC67-MA (8)	I	0.65	Undefined CarO	bla _OXA-23	+	+	+	CAZ FEP CIP MEM IPM EPM TZP TMP GEN AMK STR	≥ 32
AC70-MA (1)	N	1.55	CarOa	bla _OXA-66	+	-	-	ATM CAZ FEP CIP MEM EPM TZP TMP GEN AMK STR	1.0
AC71-MA (2)	K	0.45	Undefined CarO	bla _OXA-23	+	+	+	CAZ FEP CIP MEM IPM EPM TZP TMP GEN AMK STR	≥ 32
ATCC 19606		1.00	CarOa						0.25

^aall isolates also harboured the bla_OXA-51 gene.
^bno bla_OXA-51 was associated with ISAba1.
ATM aztreonam, CAZ ceftazidime, FEP cefepime, CIP ciprofloxacin, MEM meropenem, IPM imipenem, EPM ertapenem, TZP piperacillin/tazobactam, TMP trimethoprim, GEN gentamicin, AMK amikacin, STR streptomycin.

ISSN: 1471-2180