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Figure 3 | BMC Microbiology

Figure 3

From: Effect of spaceflight on Pseudomonas aeruginosa final cell density is modulated by nutrient and oxygen availability

Figure 3

Post-growth storage of samples at 8°C for 48 h decreases final cell density. Wild-type P. aeruginosa was cultured in mAUMg containing 5 mM phosphate at 37°C for 72 h. Final cell density was measured immediately following growth (-) or following post-growth storage at 8°C for 48 h (+) by plate counting (black bars) or FCM (gray bars). For FCM samples, fixative was added immediately following growth (-) or after post-growth storage at 8°C for 48 h (+), and cells were stored at 4°C prior to staining and assessing cell numbers. Diamonds indicate results from two-way ANOVA: ♦♦p < 0.01. Error bars, SD; N = 3.

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