Post-growth storage of samples at 8°C for 48 h decreases final cell density. Wild-type P. aeruginosa was cultured in mAUMg containing 5 mM phosphate at 37°C for 72 h. Final cell density was measured immediately following growth (-) or following post-growth storage at 8°C for 48 h (+) by plate counting (black bars) or FCM (gray bars). For FCM samples, fixative was added immediately following growth (-) or after post-growth storage at 8°C for 48 h (+), and cells were stored at 4°C prior to staining and assessing cell numbers. Diamonds indicate results from two-way ANOVA: ♦♦p < 0.01. Error bars, SD; N = 3.