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Table 4 Phenotypic characteristics allowing differentiation between Bifidobacterium mongoliense (13 strains isolated during this study and the type strain DSM 21395 T ), B. crudilactis (10 strains, including the type strain) and B. psychraerophilum LMG 21775 T

From: Detection and characterization of Bifidobacterium crudilactis and B. mongoliense able to grow during the manufacturing process of French raw milk cheeses

Characteristics BM BM BC BC BP
(13 strains, % positive responses) DSM 21395T[5] (9 strains, % positive responses; [4] LMG 23609T[4] LMG 21775T[27]
Acidification of :      
L-arabinose 100 + 0 - +
D-xylose 0 - 10 - +
α-methyl-D-mannoside 0 - 0 - +
N-acetylglu-cosamine 0 - 0 - +
Salicin 85 + 20 - +
Lactose 100 + 100 + -
melezitose 0 - 10 - +
Glycogen 92 + 10 - -
Enzymatic tests :      
α-arabinosi-dase 100 NT 0 - +
glycine arylamidase 38 NT 100 + +
Growth temperature range 10°Ca-41°Cb 15°C-40°C 5°C-45°C 4°C-45°C 4°C-42°C
Minimum growth pH c NT     4.5
DNA G + C content (mol%) 61.1 (6 strains) (SD = 0.67) 61.1 55.2 (9 strains) (SD = 0.83) 56.4 (4 exper.) (SD = 0.60) 59.2 (HPLC, Simpson et al., 2004) 55.7 (Tmd)
  1. Legend of Table 4: BM: B. mongoliense, BC: B. crudilactis, BP: B. psychraerophilum; a, growth within 14 days; b, within 8 days; c, within 48 h; d, mean of 2 experiments performed in the laboratory; NT, not tested. Other phenotypic characteristics of the French B. mongoliense strains: acidification by ≥ 90% of the strains of ribose, galactose, glucose, esculin, cellobiose, maltose, melibiose and raffinose and were positive for α-galactosidase, β-galactosidase, α-glucosidase, β-glucosidase, arginine arylamidase, proline arylamidase, phenylalanine arylamidase, tyrosine arylamidase and histidine arylamidase. None (≤ 10% of the strains) ferment glycerol, erythritol, D-arabinose, L-xylose, adonitol, β-methyl-xyloside, L-sorbose, rhamnose, dulcitol, inositol, mannitol, sorbitol, inulin, xylitol, D-lyxose, D-tagatose, D-fucose, L-fucose, D-arabitol, L-arabitol, gluconate, 2-keto-gluconate, 5-keto-gluconate. All strains (≤ 10% of the strains) were negative for urease, indole production, nitrate reduction, arginine dihydrolase, β-galactosidase-6-phosphate, β-glucuronidase, β-N-acetylglucosaminidase, acide glutamique decarboxylase, α-fucosidase, acide pyroglutamique arylamidase, glutamyl arylamidase.