Enterohepatic bacterial infections dysregulate the FGF15-FGFR4 endocrine axis

Romain, Guillaume; Tremblay, Sarah; Arena, Ellen T; Antunes, L Caetano M; Covey, Scott; Chow, Michael T; Finlay, B Brett; Menendez, Alfredo

doi:10.1186/1471-2180-13-238

BMC Microbiology

Table 1 The genes analyzed in this study and the sequences of the qPCR primer sets

From: Enterohepatic bacterial infections dysregulate the FGF15-FGFR4 endocrine axis

Gene	Official symbol	Product	Primers
Abcg5	Abcg5	ATP-binding cassette, sub-family G (WHITE), member 5	TGTCAACAGTATAGTGGCTCTG
Abcg5	Abcg5	ATP-binding cassette, sub-family G (WHITE), member 5	CGTAAAACTCATTGACCACGAG
Abcg8	Abcg8	ATP-binding cassette, sub-family G (WHITE), member 8	CTTGTCCTCGCTATAGCAACC
Abcg8	Abcg8	ATP-binding cassette, sub-family G (WHITE), member 8	TTTCCACAGAAAGTCATCAAAGC
Asbt	Slc10a2	Apical sodium-dependent bile acid transporter	ACCTTCCCACTCATCTATACTG
Asbt	Slc10a2	Apical sodium-dependent bile acid transporter	CAAATGATGGCCTGGAGTCC
Bsep	Abcb11	Bile salt export pump	CAACGCATTGCTATTGCTCGG
Bsep	Abcb11	Bile salt export pump	TAGACAAGCGATGAGCAATGAC
Cyp7a1	Cyp7a1	Cholesterol 7 alpha hydroxylase	GGGAATGCCATTTACTTGGATC
Cyp7a1	Cyp7a1	Cholesterol 7 alpha hydroxylase	TATAGGAACCATCCTCAAGGTG
Fabp6	Fabp6	Fatty acid binding protein 6	GAATTACGATGAGTTCATGAAGC
Fabp6	Fabp6	Fatty acid binding protein 6	TTGCCAATGGTGAACTTGTTGC
Fgf15	Fgf15	Fibroblast growth factor 15	AGACGATTGCCATCAAGGACG
Fgf15	Fgf15	Fibroblast growth factor 15	GTACTGGTTGTAGCCTAAACAG
FgfR4	Fgfr4	Fibroblast growth factor receptor 4	CTCGATCCGCTTTGGGAATTC
FgfR4	Fgfr4	Fibroblast growth factor receptor 4	CAGGTCTGCCAAATCCTTGTC
FXR	Nr1h4	Farnesoid X receptor (nuclear receptor subfamily 1, group H, member 4)	GTTCGGCGGAGATTTTCAATAAG
FXR	Nr1h4		AGTCATTTTGAGTTCTCCAACAC
βKlotho	Klb	Beta Klotho	AACAGCTGTCTACACTGTGGG
βKlotho	Klb	Beta Klotho	ATGGAGTGCTGGCAGTTGATC
Mdr1a	Abcb1a	ATP-binding cassette, sub-family B member 1a	CCGATAAAAGAGCCATGTTTGC
Mdr1a	Abcb1a	ATP-binding cassette, sub-family B member 1a	CTTCTGCCTGATCTTGTGTATC
Mdr1b	Abcb1b	ATP-binding cassette sub-family B member 1b	GGACCCAACAGTACTCTGATC
Mdr1b	Abcb1b	ATP-binding cassette sub-family B member 1b	ACTTCTGCCTAATCTTGTGTATC
Mdr2	Abcb4	Multidrug resistance protein 2	TTGTCAATGCTAAATCCAGGAAG
Mdr2	Abcb4	Multidrug resistance protein 2	AGTTCAGTGGTGCCCTTGATG
Mrp2	Abcc2	ATP-binding cassette, sub-family C (CFTR/MRP) member 2	GGCTCATCTCAAATCCTTTGTG
Mrp2	Abcc2	ATP-binding cassette, sub-family C (CFTR/MRP) member 2	TTTTGGATTTTCGAAGCACGGC
Mrp3	Abcc3	ATP-binding cassette, sub-family C (CFTR/MRP), member 3	GAACACGTTCGTGAGCAGCC
Mrp3	Abcc3	ATP-binding cassette, sub-family C (CFTR/MRP), member 3	ATCCGTCTCCAAGTCAATGGC
Mrp4	Abcc4	ATP-binding cassette, sub-family C (CFTR/MRP), member 4	TACAAGATGGTTCAGCAACTGG
Mrp4	Abcc4	ATP-binding cassette, sub-family C (CFTR/MRP), member 4	GTCCATTGGAGGTGTTCATAAC
Ntcp	Slc10a1	Sodium-taurocholate co-transporting polypeptide	CGTCATGACACCACACTTACTG
Ntcp	Slc10a1	Sodium-taurocholate co-transporting polypeptide	GATGGTAGAACAGAGTTGGACG
Osta	Osta	Organic solute transporter alpha	TCTCCATCTTGGCTAACAGTG
Osta	Osta	Organic solute transporter alpha	GATAGTACATTCGTGTCAGCAC
Ostb	Ostb	Organic solute transporter beta	CCACAGTGCAGAGAAAGCTGC
Ostb	Ostb	Organic solute transporter beta	ACATGCTTGTCATGACCACCAG
Shp	Nr0b2	Small heterodimer partner	AGTCTTTCTGGAGCCTTGAGC
Shp	Nr0b2	Small heterodimer partner	TTGCAGGTGTGCGATGTGGC
SrbI	Scarb1	Scavenger receptor class B type 1	GAACTGTTCTGTGAAGATGCAG
SrbI	Scarb1	Scavenger receptor class B type 1	GCGTGTAGAACGTGCTCAGG
36B4	Rplp0	Ribosomal protein, large, P0	TCTGGAGGGTGTCCGCAAC
36B4	Rplp0	Ribosomal protein, large, P0	CTTGACCTTTTCAGTAAGTGG

The top sequence of each set corresponds to the forward primer and the bottom one to the reverse. All reactions were done in 10 μl final volume with 40 cycles of 30 seconds denaturing at 95°C, 30 seconds annealing at 60°C and 30 seconds extension at 72°C (except annealing temperature for Ostβ, which was 62°C).

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ISSN: 1471-2180

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