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Table 3 The comparison of pH and temperature properties of Gal308 to other known thermostable β-galactosidases

From: Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin

β-Galactosidase and its origin

Substrate

Optimal pH

Optimal temperature

Relative activity

Reference

β-Galactosidase (T. maritima)

lactose

6.5

80°C

NT

[7]

BgaB (B.stearothermophilus)

ONPG

7.0

70°C

80% (65°C)

[8]

β-Galactosidase (S. elviae CBS8119)

ONPG

4.5-5.5

85°C

~45% (65°C)

[9]

β-Galactosidase (Rhizomucor sp.)

pNPG

4.5

60°C

NT

[11]

Bgly (A. acidocaldarius)

ONPG

5.8

70°C

~85% (65°C)

[12]

β-Galactosidase (C. saccharolyticus)

pNPG

6.0

80°C

60% (65°C)

[13]

β-Galactosidase (B. coagulans RCS3)

ONPG

6.8

50°C

~40% (60°C)

[23]

β-Galactosidase (P. woesei)

ONPG

6.6

90°C

NT

[24]

BgaA (Thermus sp. IB-21)

pNPG

5.0-6.0

90°C

90% (95°C)

[25]

Gal308 (uncultured microbes)

lactose

6.8

78°C

87.2% (65°C)

This study