BDSF attenuates the virulence of P. aeruginosa PA14 in both in vitro and in vivo model. Cytotoxicity was assayed by monitoring LDH released by the HeLa cells infected with a MOI of about 50. Experiments were performed with DMEM medium supplemented with BDSF. The data are the means of three repeats and error bars indicate the standard deviations (A). Zebra-fish model was assayed by testing the survival rate of the fish after infection with P. aeruginosa PA14, without (■) and with (□) treatment of BDSF. PBS containing relevant BDSF was injected in the same way as for a mock control (♦). The experiment was repeated three times, and a representative set of data is shown (B).