Figure 6From: Cis-2-dodecenoic acid signal modulates virulence of Pseudomonas aeruginosa through interference with quorum sensing systems and T3SSBDSF attenuates the virulence of P. aeruginosa PA14 in both in vitro and in vivo model. Cytotoxicity was assayed by monitoring LDH released by the HeLa cells infected with a MOI of about 50. Experiments were performed with DMEM medium supplemented with BDSF. The data are the means of three repeats and error bars indicate the standard deviations (A). Zebra-fish model was assayed by testing the survival rate of the fish after infection with P. aeruginosa PA14, without (■) and with (□) treatment of BDSF. PBS containing relevant BDSF was injected in the same way as for a mock control (♦). The experiment was repeated three times, and a representative set of data is shown (B).Back to article page