Figure 4

Inhibitory effect of BDSF on T3SS of P. aeruginosa at its physiological relevant level, as determined by using P exsCEBA-lacZ fusion reporter strain. Bacteria were grown in the mixture of LB broth with cell-free supernatants of B. cenocepacia J2315 wild-type (■), Δ0581 (▲), Δ0581(0581) (▼) in a ratio of 1:1 (v/v), supplemented with 5 mM NTA. The data are the means of three repeats and error bars indicate the standard deviations.