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Table 2 Designations, targets, and positions of primers for PCR analysis and Southern blot hybridization

From: Molecular analysis of subtilase cytotoxin genes of food-borne Shiga toxin-producing Escherichia coli reveals a new allelic subAB variant

Primer

Target

Sequence (5′ – 3′)

GenBank accession number

Reference

saaDF

saa

5′-CGT GAT GAA CAG GCT ATT GC-3′

AF399919

[26]

saaDR

saa

5′-ATG GAC ATG CCT GTG GCA AC-3′

AF399919

[26]

subAB-V-for

subAB

5′-CTT CCC TCA TTG CCT CAC G-3′

AY258503

This study

subAB-V-rev

subAB

5′-GGC TGG CCT GTT GTG TAA A-3′.

AY258503

This study

tia_lo

tia

5′-TCC ATG CGA AGT TGT TAT CA-3′

U20318

[15]

tia_sense

tia

5′-TTC TCT TTT TAC CCT GCT TTT TGC-3′

FJ664545

[15]

subAB-for5

subAB 1

5′-CGT ATC TGC GCC ATA TCC TG-3′

AY258503

This study

subAB-rev5

subAB 1

5′-CTG TTC CGA GCA GCC ATA TC-3′

AY258503

This study

subAB2-3′tia

subAB 2-1

5′-ACT GGC TGT TCT AAC CG-3′

AEZO02000028.1

This study

subA_out

subA 2-2

5′-GAA TCA ACA ACA GAT ACG AC-3′

AEZO02000020.1

This study

subA-L

Linkera

5′-ATG AAT GAG AGC ATC CCT-3′

AEZO02000020.1

This study

subAB5′OEP

subAB 2-2

5′-TAA TGT TTT TGA GAC GGG-3′

AEZO02000020.1

This study

subAB2-3′out

subAB 2-2

5′-AGG TCG GCT CAG TGT TC-3′

AEZO02000020.1

This study

  1. aintergenic linker between the OEP-locus and subA 2-2.