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Table 2 Designations, targets, and positions of primers for PCR analysis and Southern blot hybridization

From: Molecular analysis of subtilase cytotoxin genes of food-borne Shiga toxin-producing Escherichia coli reveals a new allelic subAB variant

Primer Target Sequence (5′ – 3′) GenBank accession number Reference
saaDF saa 5′-CGT GAT GAA CAG GCT ATT GC-3′ AF399919 [26]
saaDR saa 5′-ATG GAC ATG CCT GTG GCA AC-3′ AF399919 [26]
subAB-V-for subAB 5′-CTT CCC TCA TTG CCT CAC G-3′ AY258503 This study
subAB-V-rev subAB 5′-GGC TGG CCT GTT GTG TAA A-3′. AY258503 This study
tia_lo tia 5′-TCC ATG CGA AGT TGT TAT CA-3′ U20318 [15]
tia_sense tia 5′-TTC TCT TTT TAC CCT GCT TTT TGC-3′ FJ664545 [15]
subAB-for5 subAB 1 5′-CGT ATC TGC GCC ATA TCC TG-3′ AY258503 This study
subAB-rev5 subAB 1 5′-CTG TTC CGA GCA GCC ATA TC-3′ AY258503 This study
subAB2-3′tia subAB 2-1 5′-ACT GGC TGT TCT AAC CG-3′ AEZO02000028.1 This study
subA_out subA 2-2 5′-GAA TCA ACA ACA GAT ACG AC-3′ AEZO02000020.1 This study
subA-L Linkera 5′-ATG AAT GAG AGC ATC CCT-3′ AEZO02000020.1 This study
subAB5′OEP subAB 2-2 5′-TAA TGT TTT TGA GAC GGG-3′ AEZO02000020.1 This study
subAB2-3′out subAB 2-2 5′-AGG TCG GCT CAG TGT TC-3′ AEZO02000020.1 This study
  1. aintergenic linker between the OEP-locus and subA 2-2.