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Table 3 Rates of loss of heterozygosity in wild-type and mutant diploid strains

From: Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae

Genotype

ILOH rate (10-5)

TLOH rate (10-4)

CL rate (10-5)

Wild-type

2.5 (2.1, 3.1) [1]

0.92 (0.62, 1.2) [1]

3.0 (2.5, 3.9) [1]

rad51::LEU2/rad51::LEU2

1.2 (0.92, 2.5) [−2]

1.3 (0.38, 2) [+1.4]

54 (19, 64) [+18]

rad59::LEU2/rad59::LEU2

1.8 (1.2, 2.9) [−1.4]

1.4 (1.1, 1.9) [+1.5]

6.2 (5.8, 10.2) [+2]

rad59-Y92A/rad59-Y92A

3.2 (2.7, 4.8) [+1.3]

0.95 (0.83, 1.5) [1]

2.5 (2.0, 3.6) [−1.2]

rad59-K174A/rad59-K174A

2.0 (1.3, 3.5) [−1.3]

0.76 (0.40, 1.1) [−1.2]

5.6 (2.9, 8.4) [+1.9]

rad59-F180A/rad59-F180A

3.8 (3.1, 5.1) [+1.5]

0.82 (0.63, 1.7) [−1.1]

3.0 (1.5, 7.9) [1]

rad27::LEU2/rad27::LEU2

28 (25, 64) [+11]

34 (24, 47) [+37]

38 (29, 54) [+13]

rad27::LEU2/rad27::LEU2 rad59-Y92A/rad59-Y92A

28 (13, 56) [+11]

36 (17, 50) [+39]

29 (23, 74) [+9.7]

rad27::LEU2/rad27::LEU2 rad59-K174A/rad59-K174A

26 (22, 55) [+10]

33 (24, 39) [+36]

32 (18, 48) [+11]

rad27::LEU2/rad27::LEU2 rad59-F180A/rad59-F180A

52 (29, 76) [+21]

35 (22, 57) [+38]

57 (18,124) [+19]

  1. Rates of interstitial LOH (ILOH), terminal LOH (TLOH), and chromosome loss (CL) from a minimum of 12 independent cultures were determined as described in the Methods. The 95% confidence intervals are in parentheses. Fold decreases (−) and increases (+) from wild-type are in brackets.