The rad59 mutant alleles have distinct effects on cell cycle distribution in rad27::LEU2 mutant cells. Wild-type, single and double mutant strains were grown to mid-log phase at 30°, fixed, stained with propidium iodide, and submitted to flow cytometric analysis as described in the Methods. (A) Cell cycle profiles for wild-type and rad59 mutant strains. (B) Cell cycle profiles for rad27 single and rad27 rad59 double mutants. The distribution of cells with 1n and 2n DNA content in representative cultures of each strain are depicted. (C) Cell cycle distribution for wild-type and mutant strains. Median ratios of G1 to S + G2/M cells from a minimum of five independent cultures are indicated for each strain, and 95% confidence intervals are plotted.