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Figure 1 | BMC Microbiology

Figure 1

From: Alleles of the homologous recombination gene, RAD59, identify multiple responses to disrupted DNA replication in Saccharomyces cerevisiae

Figure 1

The rad59 mutant alleles have distinct effects on survival in cells that are defective for lagging strand synthesis. Diploid strains heterozygous at the RAD27 (rad27::LEU2/RAD27) and RAD59 (rad59/RAD59) loci were sporulated and tetrads dissected onto YPD medium. The resulting colonies were examined after 72 h of growth at 30°. Colonies from five representative tetrads from each strain are displayed. The genotype of each colony was determined by PCR as described in the Methods. In the inverted image, colonies possessing a rad27::LEU2 allele are boxed in black, and those possessing a rad59 allele are circled in white.

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