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Table 2 Oligonucleotide primers used in RDA assay and qRT-PCR

From: Transcriptional profile of Paracoccidioides induced by oenothein B, a potential antifungal agent from the Brazilian Cerrado plant Eugenia uniflora

Sequence name

Forward primer (5′- 3′)

Reverse primer (5′- 3′)

Reaction

cDNA

AGCAGTGGTATCAACGACAGAGTACGCGGG

 

Synthesis of the first-strand for RDA

CDS

AAGCAGTGGTATCAACGCAGAGTACT(30)N1N

 

Synthesis of the first-strand for RDA

PCRII

AAGCAGTGGTATCAACGCAGAGT

 

Synthesis of the first-strand for RDA

JBam12, 24

GATCCGTTCATG

ACCGACGTCGACTATCCATGAACG

Adapter 1 (RDA)

NBam12,24

GATCCTCCCTCG

AGGCAACTGTGCTATCCGAGGGAG

Adapter 2 (RDA)

T7

GTAATACGACTCACTATAGGGC

 

Sequencing

Oligo (dT)15

AAGCAGTGGTATCAACGCAGAGTACT(30)N1N3′

 

Synthesis of the first-strand for qRT-PCR

CAP20

CCTTCACGAACTCGCCACTAT

TCGCTGCTTAGGGAGTCTGC

qRT-PCR

FKS1

GACAACAGAGGGTATAATGGG

GCCATATTGATAGCCTGCAGC

qRT-PCR

GLN1

CGATCAAAAACAAAGACCCT

GGTCTGGGTACATGGCAAC

qRT-PCR

KRE6

GGTATATGCCTAACTTTGAATTC

GCGTAGACTTGATACTCTTTTG

qRT-PCR

GAC1

AGTACTGCTTCTATGGATCTTC

ACTATTTCCTGGGGTCGTTG

qRT-PCR

MFS

CTAATTATGTTCTTTTGGGGTAC

GCATCGCCTATACCAACAAGA

qRT-PCR

PAL1

TGCTGCGGAACTCTTTGA

GGGCTTATCGTCGGAGAGTC

qRT-PCR

ERG3

CACTTGGATCTTCGGCCTAAT

TGCATAGCCACGGACTTCGA

qRT-PCR

  1. (N1 = A, G or C/N = A, C, G or T).