Plasmids | Relevant characteristics | Source/Reference |
---|---|---|
pGEM-T Easy | ampRoripUC (Cloning vector) | Promega |
pMV261 | kanR (Mycobacterial shuttle non-integrative vector) | Stover et al., 1991 [14] |
pDS1 | pGEM-T Easy containing glnA1 coding sequence with native promoter | This work |
pDS2 | pMV261 containing glnA1 coding sequence with native promoter | This work |
pDS3 | pGEM-T Easy containing glnA1 coding sequence with P1 promoter | This work |
pDS4 | pMV261 containing glnA1 coding sequence with P1 promoter | This work |
pDS5 | pMV261 containing glnA1 coding sequence with P2 promoter | This work |
Strains | Relevant characteristics | Source/Reference |
DH5α | supE44 ΔlacU(Φ80lacZΔM15) hsdR17 rec1 endA1 gyrA96 thi-1 relA1 | Novagen |
M. bovis AN5 | Wild Type | ATCC |
M. smegmatis mc2 | Wild Type | ATCC |
MSFP | M. smegmatis containing pDS2 | This work |
MSP1 | M. smegmatis containing pDS4 | This work |
MSP2 | M. smegmatis containing pDS5 | This work |