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Table 1 Plasmids and strains used in this study

From: Poly-L-glutamate/glutamine synthesis in the cell wall of Mycobacterium bovisis regulated in response to nitrogen availability

Plasmids Relevant characteristics Source/Reference
pGEM-T Easy ampRoripUC (Cloning vector) Promega
pMV261 kanR (Mycobacterial shuttle non-integrative vector) Stover et al., 1991 [14]
pDS1 pGEM-T Easy containing glnA1 coding sequence with native promoter This work
pDS2 pMV261 containing glnA1 coding sequence with native promoter This work
pDS3 pGEM-T Easy containing glnA1 coding sequence with P1 promoter This work
pDS4 pMV261 containing glnA1 coding sequence with P1 promoter This work
pDS5 pMV261 containing glnA1 coding sequence with P2 promoter This work
Strains Relevant characteristics Source/Reference
DH5α supE44 ΔlacU(Φ80lacZΔM15) hsdR17 rec1 endA1 gyrA96 thi-1 relA1 Novagen
M. bovis AN5 Wild Type ATCC
M. smegmatis mc2 Wild Type ATCC
MSFP M. smegmatis containing pDS2 This work
MSP1 M. smegmatis containing pDS4 This work
MSP2 M. smegmatis containing pDS5 This work
  1. Selection marker resistant to Ampicillin (ampR) and Kanamycin (kanR).