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Table 1 Plasmids and strains used in this study

From: Poly-L-glutamate/glutamine synthesis in the cell wall of Mycobacterium bovisis regulated in response to nitrogen availability

Plasmids

Relevant characteristics

Source/Reference

pGEM-T Easy

ampRoripUC (Cloning vector)

Promega

pMV261

kanR (Mycobacterial shuttle non-integrative vector)

Stover et al., 1991 [14]

pDS1

pGEM-T Easy containing glnA1 coding sequence with native promoter

This work

pDS2

pMV261 containing glnA1 coding sequence with native promoter

This work

pDS3

pGEM-T Easy containing glnA1 coding sequence with P1 promoter

This work

pDS4

pMV261 containing glnA1 coding sequence with P1 promoter

This work

pDS5

pMV261 containing glnA1 coding sequence with P2 promoter

This work

Strains

Relevant characteristics

Source/Reference

DH5α

supE44 ΔlacU(Φ80lacZΔM15) hsdR17 rec1 endA1 gyrA96 thi-1 relA1

Novagen

M. bovis AN5

Wild Type

ATCC

M. smegmatis mc2

Wild Type

ATCC

MSFP

M. smegmatis containing pDS2

This work

MSP1

M. smegmatis containing pDS4

This work

MSP2

M. smegmatis containing pDS5

This work

  1. Selection marker resistant to Ampicillin (ampR) and Kanamycin (kanR).