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Table 4 Examples of scattergrams of inhibition zone measurements with calliper, the Sirscan system adaped on-screen by the human eye and the Sirscan fully automated mode

From: Standardisation of disk diffusion results for antibiotic susceptibility testing using the sirscan automated zone reader

Nitrofurantoin, E. coli ATCC 25922

 

Diameter (mm)

15

16

17

18

19

20

21

22

23

24

          

   Sirscan fully automated

  

9

10

                

   Sirscan on-screen adjusted

 

6

4

5

2

2

              

   Calliper

3

3

4

3

5

1

              

Ertapenem, E. coli ATCC 25922

 

Diameter (mm)

28

29

30

31

32

33

34

35

36

37

38

         

   Sirscan fully automated

     

3

7

9

            

   Sirscan on-screen adjusted

    

1

 

4

6

2

3

3

         

   Calliper

1

  

1

1

4

3

1

5

3

          

Trimethoprim-Sulfamethoxazole, S. aureus ATCC 29213

 

Diameter (mm)

18

19

20

21

22

23

24

25

26

27

28

29

30

31

32

33

34

35

36

37

   Sirscan fully automated

          

4

6

7

2

      

   Sirscan on-screen adjusted

          

1

4

3

7

4

     

   Calliper

2

1

1

  

1

 

1

2

3

2

1

2

1

1

 

1

   

Amikacin, S. aureus ATCC 29213

 

Diameter (mm)

17

18

19

20

21

22

23

24

25

           

   Sirscan fully automated

     

7

12

             

   Sirscan on-screen adjusted

     

1

6

8

4

           

   Calliper

 

1

   

5

3

7

3

           
  1. Measurements were done independently and double-blinded by 19 experienced persons (technicians and laboratory physicians) with the same disk diffusion plates of EUCAST quality control strains of S. aureus ATCC 29213, and E. coli ATCC 25922. Measurements of the Sirscan fully automated mode comprise 19 independent measurements of the panels. EUCAST quality control ranges are indicated in italics.