Table 2 Comparison of experimentally determined eliminations from N-terminal AspN digested/tryptic peptides of LprF, LpqH, LpqL and LppX in the MALDI-TOF/TOF spectra of BCG parental and Δ lnt mutant strain with theoretically calculated eliminations

O-linked palmitoyl (C16)

Palmitic acid

256.24

256.5

-

256.3

256.3

n.d. *

-

-

256.2

256.1

256.3

256.3

n.d. *

O-linked oleyl (C18)

Oleic acid

282.24

-

-

-

-

n.d. *

-

-

-

282.4

-

-

n.d. *

O-linked tuberculostearyl (C19)

Tuberculostearic acid

298.29

-

-

298.3

298.3

n.d. *

-

-

-

-

298.3

298.4

n.d. *

N-linked palmitoyl (C16) + Didehydroalanine

Palmitamide + Didehydroalanine

307.26

-

306.6

-

-

n.d. *

-

-

-

-

-

-

n.d. *

N-linked tuberculostearyl (C19) + Didehydroalanine

Tuberculostearinamide + Didehydroalanine

349.31

349.8

-

-

-

n.d. *

-

-

-

-

-

-

n.d. *

Diacylglyceryl (C16/C16)

Diacylhioglyceryl (C16/C16)

584.44

-

-

-

-

n.d. *

583.3

-

-

-

-

-

n.d. *

Diacylglyceryl (C16/C18)

Diacylhioglyceryl (C16/C18)

610.52

-

-

-

-

n.d. *

-

-

-

-

-

-

n.d. *

Diacylglyceryl (C16/C19)

Diacylhioglyceryl (C16/C19)

626.53

625.9

626.7

626.7

626.6

n.d. *

-

626.7

-

-

626.6

626.7

n.d. *

C16 fatty acid α-thioglyceryl ester

328.24

-

-

328.4

328.3

n.d. *

-

-

-

-

-

n.d. *

C19 fatty acid α-thioglyceryl ester

370.29

-

-

370.5

370.3

n.d. *

-

369.8

-

-

-

370.4

n.d. *

Hexose

Hexose

160.76

161.62

-

-

-

n.d. *

-

162.9

-

-

-

-

n.d. *