Table 1 Strains, plasmids and oligonucleotides used in this study
From: The SseC translocon component in Salmonella enterica serovar Typhimurium is chaperoned by SscA
Strains, plasmids and primers | Description | Reference or source |
|---|---|---|
Strains | Â | Â |
SL1344 | Salmonella enterica serovar Typhimurium wild type | Our collection |
∆sscA | SL1344, deletion of sscA | This study |
∆sseC | SL1344, deletion of sseC | This study |
SL1344 RES | SL1344, ushA::Cm; CmR (10 μg/ml) | [29] |
Plasmids | Â | Â |
pKD4 | oriRγ, KanR cassette flanked by FRT sites | [26] |
pKD46 | RepA1019(Ts), λ, γ, β and exo expressed from ParaBAD, AmpR | [26] |
pFLAG-CTC | Cytoplasmic expression of C-terminal FLAG fusion protein under control of the Ptac, AmpR | Sigma |
psscA-FLAG | pFLAG-CTC, expresses in-frame fusion of SscA-FLAG | This study |
psseC-FLAG | pFLAG-CTC, expresses in-frame fusion of SseC-FLAG | This study |
pACYC184 | General cloning, TetR, CmR | Our collection |
psscA-6His | pACYC; expresses in-frame fusion of SscA-6His | This study |
pBAD24 | Arabinose-inducible gene expression, pBR322ori, PBAD, rrnBT1,2, araC, AmpR | [27] |
psscA | sseA-sscA with endogenous sseA promoter in pWSK29, pSC101ori, AmpR | This study |
Primers | Â | Â |
sseC lambda-F | cgaattcacagtaatagcgacagcgccgcaggagtaaccgccttaacacagtgtaggctggagctgcttcg | Â |
sseC lambda-R | gcgatagccagctattctcgcctgaacgctactatagtgatcaatggtatcatatgaatatcctcctta | Â |
sscA lambda-F | gacccgaccctacaacaggcacatgacacgatgcggtttttccggcgtgggtgtaggctggagctgcttcg | Â |
sscA lambda-R | gtcagaaagttgctgtaacatcttttctgcacgctgtcggagaatttgatcatatgaatatcctcctta | Â |
sscA-FLAG-F | gcttaaagcttatgaaaaaagacccgaccct | Â |
sscA-FLAG-R | tatctgtcgacgctcctgtcagaaagttgct | Â |
sseC-FLAG-F | ggtcacatatgatgaatcgaattcacagtaa | Â |
sseC-FLAG-R | ggtcagtcgacagcgcgatagccagctattc | Â |
sscA-HIS-F | gtcaggctagcaggaggatgcatcaccatcaccatcacatgaaaaaagacccgaccc | Â |
sscA-HIS-R | gtcagaagcttttagctcctgtcagaaagttg | Â |
sscA-F | acgcgtcgacacaggatccgcagcaatatc | Â |
sscA-R | gctctagacccctaaatatgcaggctca | Â |