Performance of five molecular methods for monitoring Arcobacter spp

Levican, Arturo; Figueras, María José

doi:10.1186/1471-2180-13-220

BMC Microbiology

Table 2 Identification results obtained for 95 strains of 17 Arcobacter spp. when using the five different PCR identification methods

From: Performance of five molecular methods for monitoring Arcobacter spp

Species	Strains^a	Houf *et al*. [14]	Kabeya *et al*. [15]	Figueras *et al.* [18]^b	Pentimalli *et al*. [16]	Douidah *et al.* [9]
Species	Strains^a	Houf *et al*. [14]	Kabeya *et al*. [15]	Figueras *et al.* [18]^b	Pentimalli *et al*. [16]	De Smet *et al*. [17]^c
*A. butzleri* (Ab)	21	21 Ab	1 Ab^d	21 Ab	21 Ab	21 Ab
			15 Ab + Acr1B^e
			5 NA^f
*A. cryaerophilus* (Acr)	19	19 Acr	19 Acr	12 Acr	19 Acr	19 Acr
*A. cryaerophilus* (Acr)	19	19 Acr	19 Acr	7 Ab	19 Acr	19 Acr
Acr1A (n=6)			5 Acr1A^d	6 Acry1A^d
Acr1A (n=6)			1 Acr1B	6 Acry1A^d
Acr1B (n=6)			5 Acr1B	6 Acry1B
Acr1B (n=6)			1 Acr1A	6 Acry1B
*A. skirrowii* (Aski)	5	5 Aski	5 Aski	5 Aski	3 Aski^d,g	5 Aski
*A. skirrowii* (Aski)	5	5 Aski	5 Aski	5 Aski	2 NA	5 Aski
*A. nitrofigilis* (Anit)	5	5 Aski	4 Acr1B^d	5 Anit	2 Ab	NA
			1 Ab + Acr1B		2 Acr
			1 Ab + Acr1B		3 NA*^d
*A. halophilus* (Ahalo)	1	1 Aski + Acr	1 Aski	1 Ahalo	NA*	NA
*A. cibarius* (Acib)	8	8 NA	3 Aski^d	8 Acib	8 Ab	8 Acib
			5 Aski + Acr1B		8 Acib
			5 Aski + Acr1B		3 Aski
*A. thereius* (Ather)	5	5 Acr	1 Ab	5 Ab	5 NA*	5 Ather
			2 Ab + Acr1B^d
			1 Acr1B
			1 NA
*A. mytili* (Amyt)	3	3 Aski	3 Aski	3 Amyt	3 NA*	3 NA
*A. marinus* (Amar)	1	1 Acr	1 NA	1 Amar^h	1 Ab	1 NA
*A. molluscorum* (Amoll)	3	3 Aski + Acr	3 NA	3 Amoll	3 NA*	3 NA
*A. defluvii* (Adef)	11	11 Acr	11 Ab	11 Adef	11 NA*^d	11 Ab
*A. trophiarum* (Atroph)	3	3 Acr	2 Ab^d	3 Ab	3 NA*	3 Atroph
*A. trophiarum* (Atroph)	3	3 Acr	1 NA	3 Ab	3 NA*	3 Atroph
*A. ellisii* (Aelli)	3	3 Acr	3 Acr1A + Acr1B	3 Aelli	2 Aski	1 Ab
*A. ellisii* (Aelli)	3	3 Acr	3 Acr1A + Acr1B	3 Aelli	1 NA*^d	2 Ab +Acr^d
*A. bivalviorum* (Abiv)	3	3 Acr	3 Acr1B	3 Abiv	3 NA	3 NA
*A. venerupis* (Aven)	1	1 Acr	1 Ab	1 Aven^h	1 Ab	1 Ab
*A. cloacae* (Acloa)	2	2 Acr	2 Ab + Acr1B	2 Acloa	2 NA*	2 NA
*A. suis* (Asuis)	1	1 Acr	1 Acr1A	1 Adef	1 NA	1 Ab
Correctly identified strains		53 (55.8%)	31 (32.6%)	79 (83.2%)	79 (83.2%)	79 (83.2%)

^aAll strains were identified using the RFLP method of Figueras et al. [19] that had been specifically designed to recognize all species. The new species Arcobacter anaerophilus was not tested as it had only recently been described [8]. However, a computer simulation of the digestion of the 16S rRNA gene sequence of the type strain of this species (Accession number FR686494) using the MseI endonuclease [18, 19] showed a species-specific RFLP pattern (658, 138, 60, 52, 41, 34, 28, 12, 3 bp).
^bAs this method was designed for A. butzleri, A. cryaerophilus, A. cibarius, A. skirrowii, A. nitrofigilis and A. halophilus[18], the results for strains of other species were interpreted based on the RFLP patterns described in subsequent publications [5–7, 23–25].
^cThe method designed by De Smet et al.[17] only detects or identifies A. trophiarum, and was intended to complement the m-PCR of Douidah et al.[9]. Therefore, they are grouped together as a single method.
^dResult obtained for the type strain.
^eSpecies A + species B refers to the fact that the expected amplicon for species A and B were obtained in the same reaction.
^fNA or NA*: No amplification of a band of the expected size, or (*) band/s of another size were obtained.
^gWhen different results were obtained using the four individual PCR reactions designed by Pentimalli et al. [16] for A. butzleri, A. cryaerophilus, A. skirrowii, and A. cibarius, they are shown on separate lines.
^hA. venerupis produced a pattern very similar to that of A. marinus[19].

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ISSN: 1471-2180

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