Diffusely adherent Escherichia colistrains isolated from children and adults constitute two different populations

Mansan-Almeida, Rosane; Pereira, Alex Leite; Giugliano, Loreny Gimenes

doi:10.1186/1471-2180-13-22

Table 6 Primers used in polymerase chain reaction analysis

From: Diffusely adherent Escherichia colistrains isolated from children and adults constitute two different populations

Gene	Locus description	Primer sequence	Fragment length	Annealing temperature	Reference
*afa* B-C	Conserved region of Afa/Dr operons	5Â´ CTGGGCAGCAAACTGATAACTCTC 3Â´	750 pb	62°C	[75]
*afa* B-C	Conserved region of Afa/Dr operons	5Â´ CATCAAGCTGTTTGTTCGTCCGCCG 3Â´	750 pb	62°C	[75]
*afaE* -1	Afa-I afimbrial adhesin	5Â´ CGAAAACGGCACTGACAAG 3Â´	230 pb	61°C	[19]
*afaE* -1	Afa-I afimbrial adhesin	5Â´ AGGCTTCCGTGAATACAACC 3Â´	230 pb	61°C	[19]
*afaE* -2	Afa-II afimbrial adhesin	5Â´ TTAGACCGTACTGTTGTGTTACC 3	375 pb	48°C	[42]
*afaE* -2	Afa-II afimbrial adhesin	5Â´ TTTCCCAGTAGACTGGAATGAAGC 3Â´
*afaE* -3 */dre*	Afa-III afimbrial adhesin/Dr afimbrial adhesin	5Â´ TTAGACCGTACTGTTGTGTACC 3Â´	408 pb	65°C	[76]
*afaE* -3 */dre*	Afa-III afimbrial adhesin/Dr afimbrial adhesin	5Â´ ACCATTGTCGGTCGTCCAGGC 3Â´	408 pb	65°C	[76]
*afaE* -5	Afa-V afimbrial adhesin	5Â´ TTAGACCGTACTGTTGTGTTACC Â´	429 pb	48°C	[42]
*afaE* -5	Afa-V afimbrial adhesin	5Â´ AGCATCGGCGCGGTATACGGT 3Â´	429 pb	48°C	[42]
*daa* E	F1845 fimbrial adhesin	5Â´ TGACTGTGACCGAAGAGTGC 3Â´	380 pb	48°	[19]
*daa* E	F1845 fimbrial adhesin	5Â´ TTAGTTCGTCCAGTAACCCCC 3Â´	380 pb	48°	[19]
*Sat*	Secreted auto transported toxin	5Â´ GCAGCAAATATTGATATATCA 3Â´	630 pb	57°C	[21]
*Sat*	Secreted auto transported toxin	5Â´ GTTGTTGACCTCAGCCAAGGAA 3Â´	630 pb	57°C	[21]
*escJ*	Type Three Secretion System	5Â´ CACTAAGCTCGATATATAGAACCC 3’	826 pb	54°C	[20]
*escJ*	Type Three Secretion System	5’ GTCAATGTTGATGTCGTATCTAAG 3’	826 pb	54°C	[20]
*escV*	Type Three Secretion System	5’ GATGACATCATGAATAAACTC 3’	2130 pb	54°C	[20]
*escV*	Type Three Secretion System	5’ GCCTTCATATCTGGTAGAC 3’	2130 pb	54°C	[20]
*traA*	Pilin	5’ AAGTGTTCAGGGTGCTTCTG 3’	385 pb	60°C	[28]
*traA*	Pilin	5’ TATTCTCGTCTCCCGACATC 3’	385 pb	60°C	[28]
*eae*	intimin	5’ CCCGAATTCGGCACAAGCATAAGC 3’	881 pb	52°C	[77]
		5’ CCCGGATCCGTCTCGCCAGTATTCG3’

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