Titration method | Virus | Infectious / inactived | Dye | Triton ×100 | Tween 20 | IGEPAL CA-630 | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
0.1% | 0.5% | 1% | 0.1% | 0.5% | 1% | 0.1% | 0.5% | 1% | ||||
RT-qPCR | HAV | Infectious | EMA (20 μM) | 0.03 ± 0.07 | −0.06 ± 0.06 | −0.05 ± 0.05 | −0.02 ± 0.09 | −0.07 ± 0.09 | −0.02 ± 0.06 | 0.02 ± 0.13 | −0.02 ± 0.05 | −0.04 ± 0.09 |
Inactived | −2.42 ± 0.04 | −2.52 ± 0.10 | −2.48 ± 0.01 | −1.70 ± 0.05 | −1.88 ± 0.29 | −1.89 ± 0.08 | −2.23 ± 0.41 | −2.68 ± 0.01 | −2.42 ± 0.07 | |||
Infectious | PMA (50 μM) | −0.07 ± 0.02 | −0.07 ± 0.02 | 0.00 ± 0.02 | −0.05 ± 0.06 | −0.12 ± 0.07 | −0.09 ± 0.09 | −0.06 ± 0.08 | −0.04 ± 0.05 | −0.07 ± 0.10 | ||
Inactived | −2.34 ± 0.27 | −2.49 ± 0.25 | −2.51 ± 0.23 | −1.74 ± 0.07 | −1.70 ±0.09 | −1.70 ± 0.11 | −2.42 ± 0.27 | −2.49 ± 0.34 | −2.34 ± 0.19 | |||
RV (SA11) | Infectious | EMA (20 μM) | −0.80 ± 0.10 | −0.77 ± 0.08 | 0.47 ± 0.11 | 0.75 ± 0.14 | −0.72 ± 0.07 | −0.68 ± 0.09 | −0.79 ± 0.07 | −0.47 ± 0.09 | −0.71 ± 0.09 | |
Inactived | −1.66 ± 0.09 | 1.43 ± 0.15 | −1.14 ± 0.28 | −1.18 ± 0.17 | −1.89 ± 0.77 | −1.28 ± 0.20 | −1.30 ± 0.13 | −1.28 ± 0.30 | −0.81 ± 0.27 | |||
Infectious | PMA (50 μM) | −0.74 ± 0.15 | −0.77 ± 0.16 | −0.91 ± 0.20 | 0.80 ± 0.11 | −0.76 ± 0.20 | −0.80 ± 0.20 | −0.72 ± 0.14 | 0.71 ± 0.23 | −0.81 ± 0.18 | ||
Inactived | −1.34 ± 0.18 | −1.29 ± 0.13 | −1.33 ± 0.22 | −1.30 ± 0.15 | −1.39 ± 0.16 | −1.31 ± 0.49 | −1.31 ± 0.27 | −1.35 ± 0.25 | −1.14 ± 0.39 | |||
RV (Wa) | Infectious | EMA (20 μM) | −0.39 ± 0.07 | −0.24 ± 0.13 | −0.15 ± 0.10 | −0.41 ± 0.06 | −0.13 ± 0.13 | −0.37 ± 0.17 | −0.28 ± 0.22 | −0.21 ± 0.02 | 0.36 ± 0.13 | |
Inactived | −1.21 ± 0.14 | −0.68 ± 0.12 | −0.40 ± 0.16 | −1.01 ± 0.19 | −0.88 ± 0.15 | −0.58 ± 0.16 | −0.82 ± 0.43 | −0.71 ± 0.08 | −0.14 ± 0.13 | |||
Infectious | PMA (75 μM) | −0.57 ± 0.14 | −0.61 ± 0.18 | −0.61 ± 0.13 | −0.58 ± 0.15 | −0.58 ± 0.11 | −0.64 ± 0.14 | −0.60 ± 0.16 | −0.58 ± 0.15 | −0.70 ± 0.16 | ||
Inactived | −1.23 ± 0.08 | −1.11 ± 0.04 | −1.20 ± 0.18 | −1.21 ± 0.08 | −1.15 ± 0.09 | −1.15 ± 0.17 | −1.21 ± 0.08 | −1.15 ± 0.18 | −1.23 ± 0.08 | |||
Cell culture | HAV | Infectious | None | 0.09 ± 0.22 | −0.03 ± 0.17 | 0.02 ± 0.21 | 0.11 ± 0.11 | 0.16 ± 0.06 | 0.04 ± 0.25 | 0.06 ± 0.17 | −0.01 ± 0.01 | 0.14 ± 0.09 |