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Table 3 Influence of combined dyes and surfactants on viruses

From: Discrimination of infectious hepatitis A virus and rotavirus by combining dyes and surfactants with RT-qPCR

Titration method

Virus

Infectious / inactived

Dye

Triton ×100

Tween 20

IGEPAL CA-630

0.1%

0.5%

1%

0.1%

0.5%

1%

0.1%

0.5%

1%

RT-qPCR

HAV

Infectious

EMA (20 μM)

0.03 ± 0.07

−0.06 ± 0.06

−0.05 ± 0.05

−0.02 ± 0.09

−0.07 ± 0.09

−0.02 ± 0.06

0.02 ± 0.13

−0.02 ± 0.05

−0.04 ± 0.09

Inactived

−2.42 ± 0.04

−2.52 ± 0.10

−2.48 ± 0.01

−1.70 ± 0.05

−1.88 ± 0.29

−1.89 ± 0.08

−2.23 ± 0.41

−2.68 ± 0.01

−2.42 ± 0.07

Infectious

PMA (50 μM)

−0.07 ± 0.02

−0.07 ± 0.02

0.00 ± 0.02

−0.05 ± 0.06

−0.12 ± 0.07

−0.09 ± 0.09

−0.06 ± 0.08

−0.04 ± 0.05

−0.07 ± 0.10

Inactived

−2.34 ± 0.27

−2.49 ± 0.25

−2.51 ± 0.23

−1.74 ± 0.07

−1.70 ±0.09

−1.70 ± 0.11

−2.42 ± 0.27

−2.49 ± 0.34

−2.34 ± 0.19

RV (SA11)

Infectious

EMA (20 μM)

−0.80 ± 0.10

−0.77 ± 0.08

0.47 ± 0.11

0.75 ± 0.14

−0.72 ± 0.07

−0.68 ± 0.09

−0.79 ± 0.07

−0.47 ± 0.09

−0.71 ± 0.09

Inactived

−1.66 ± 0.09

1.43 ± 0.15

−1.14 ± 0.28

−1.18 ± 0.17

−1.89 ± 0.77

−1.28 ± 0.20

−1.30 ± 0.13

−1.28 ± 0.30

−0.81 ± 0.27

Infectious

PMA (50 μM)

−0.74 ± 0.15

−0.77 ± 0.16

−0.91 ± 0.20

0.80 ± 0.11

−0.76 ± 0.20

−0.80 ± 0.20

−0.72 ± 0.14

0.71 ± 0.23

−0.81 ± 0.18

Inactived

−1.34 ± 0.18

−1.29 ± 0.13

−1.33 ± 0.22

−1.30 ± 0.15

−1.39 ± 0.16

−1.31 ± 0.49

−1.31 ± 0.27

−1.35 ± 0.25

−1.14 ± 0.39

RV (Wa)

Infectious

EMA (20 μM)

−0.39 ± 0.07

−0.24 ± 0.13

−0.15 ± 0.10

−0.41 ± 0.06

−0.13 ± 0.13

−0.37 ± 0.17

−0.28 ± 0.22

−0.21 ± 0.02

0.36 ± 0.13

Inactived

−1.21 ± 0.14

−0.68 ± 0.12

−0.40 ± 0.16

−1.01 ± 0.19

−0.88 ± 0.15

−0.58 ± 0.16

−0.82 ± 0.43

−0.71 ± 0.08

−0.14 ± 0.13

Infectious

PMA (75 μM)

−0.57 ± 0.14

−0.61 ± 0.18

−0.61 ± 0.13

−0.58 ± 0.15

−0.58 ± 0.11

−0.64 ± 0.14

−0.60 ± 0.16

−0.58 ± 0.15

−0.70 ± 0.16

Inactived

−1.23 ± 0.08

−1.11 ± 0.04

−1.20 ± 0.18

−1.21 ± 0.08

−1.15 ± 0.09

−1.15 ± 0.17

−1.21 ± 0.08

−1.15 ± 0.18

−1.23 ± 0.08

Cell culture

HAV

Infectious

None

0.09 ± 0.22

−0.03 ± 0.17

0.02 ± 0.21

0.11 ± 0.11

0.16 ± 0.06

0.04 ± 0.25

0.06 ± 0.17

−0.01 ± 0.01

0.14 ± 0.09

  1. Quantification by RT-qPCR assays A after monoazide treatment combined with surfactants (Triton ×100, Tween-20, IGEPAL CA-630) of 105 TCID50 of RV (SA11), 103 TCID50 of RV (Wa) and 6× 104 PFU of HAV, infectious or inactivated at 80°C for 10 minutes, and titration by cell culture of 6× 104 PFU of infectious HAV treated with surfactants. Mean values ± SD (n=3).