Skip to main content

Table 2 Influence of dye concentration on viruses

From: Discrimination of infectious hepatitis A virus and rotavirus by combining dyes and surfactants with RT-qPCR

Titration method Virus Infectious / inactived PMA (μM) EMA (μM)
5 20 50 75 100 5 20 50 75 100
RT-qPCR HAV Infectious 0.03 ± 0.08 0.02 ± 0.08 −0.03 ± 0.02 −0.08 ± 0.01 −0.02 ± 0.05 −0.10 ± 0.17 −0.04 ± 0.02 −0.07 ± 0.07 −0.05 ± 0.05 −0.09 ± 0.03
  Inactived −0.88 ± 0.12 −1.01 ± 0.08 −1.06 ± 0.11 −1.13 ± 0.09 −1.14 ± 0.09 −1.24 ± 0.13 −1.75 ± 0.91 −1.31 ± 0.28 −1.25 ± 0.24 −1.17 ± 0.23
  RV (SA11) Infectious −0.28 ± 0.38 −0.32 ± 0.44 −0.30 ± 0.33 −0.68 ± 0.41 −0.51 ± 0.28 −0.70 ± 0.12 −0.70 ± 0.30 −0.71 ± 0.08 −0.75 ± 0.09 −0.72 ± 0.09
  Inactived −1.16 ± 0.68 −1.45 ± 0.78 −1.60 ± 0.57 −1.70 ± 0.40 −1.71 ± 0.50 −1.12 ± 0.31 −1.13 ± 0.19 −1.05 ± 0.33 −1.06 ± 0.24 −1.07 ± 0.07
  RV (Wa) Infectious 0.05 ± 0.09 −0.38 ± 0.34 −0.63 ± 0.02 −0.62 ± 0.14 −0.52 ± 0.15 −0.19 ± 0.05 −0.50 ± 0.20 −0.96 ± 0.31 −1.12 ± 0.16 −1.15 ± 0.13
  Inactived −0.24 ± 0.65 −0.62 ± 0.27 −1.00 ± 0.15 −1.44 ± 0.18 −1.45 ± 0.29 −0.52 ± 0.76 −1.51 ± 0.26 −1.81 ± 0.06 −1.72 ± 0.19 −1.48 ± 0.18
  1. Quantification by RT-qPCR assays A after monoazide treatment of 105TCID50 of RV (SA11), 103 TCID50 of RV (Wa) and 6× 104 PFU of HAV, infectious or inactivated at 80°C for 10 minutes. Mean values ± SD (n=3).