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Table 1 Binding of dyes to purified viral RNA

From: Discrimination of infectious hepatitis A virus and rotavirus by combining dyes and surfactants with RT-qPCR

[Dye] μM HAV RV (Wa) RV (SA11)
PMA EMA PMA EMA PMA EMA
A       
10 −0.09 ± 0.11 −0.12 ± 0.09 −0.87 ± 0.30 −0.52 ± 0.19 −1.47 ± 1.27 −0.41 ± 0.27
20 −1.59 ± 0.74 −0.21 ± 0.27 −1.87 −1.18 ± 0.46 −2.51 ± 0.69 −0.31 ± 0.31
50 < LOD −0.99 ± 0.51 < LOD < LOD < LOD −0.47 ± 0.15
100 < LOD < LOD < LOD < LOD < LOD −0.44 ± 0.47
200 < LOD < LOD < LOD < LOD < LOD −0.30 ± 0.41
B       
0 −0.33 ± 0.10 −0.33 ± 0.10 −0.49 ± 0.51 −0.49 ± 0.51 −0.07 ± 0.26 −0.07 ± 0.26
10 −0.55 ± 0.13 −0.41 ± 0.26 −0.39 ± 0.11 −0.16 ± 0.06 −0.51 ± 0.16 −0.32 ± 0.32
20 −0.25 ± 0.27 0.37 ± 0.05 −0.27 ± 0.22 −0.37 ± 0.12 −0.68 ± 0.49 −0.28 ± 0.23
50 0.32 ± 0.26 0.43 ± 0.51 −0.34 ± 0.09 −0.23 ± 0.20 −1.60 −0.32 ± 0.23
100 −0.54 ± 0.01 0.03 ± 0.14 −0.38 ± 0.18 0.35 ± 0.24 < LOD 0.52 ± 0.23
200 −0.36 ± 0.13 0.35 ± 0.24 −0.30 ± 0.20 −0.47 ± 0.35 < LOD −0.34 ± 0.16
C       
0 −0.33 ± 0.10 −0.33 ± 0.10 −0.49 ± 0.51 −0.49 ± 0.51 −0.07 ± 0.26 −0.07 ± 0.26
10 −2.65 ± 0.51 −0.96 ± 0.27 −1.27 ± 0.12 −0.59 ± 0.24 −1.41 ± 0.51 −0.79 ± 0.50
20 −2.27 ± 0.46 −1.08 ± 0.48 −1.33 ± 0.13 −0.07 ± 0.50 −1.48 ± 0.55 −0.64 ± 0.66
50 −3.16 ± 0.77 −1.16 ± 0.21 −1.75 ± 0.11 −0.62 ± 0.38 −2.96 ± 1.38 −1.22 ± 0.67
100 −2.47 ± 0.37 −1.56 ± 0.33 −2.20 ± 0.50 −1.01 ± 0.11 −3.58 ± 0.65 −2.06 ± 1.63
200 −2.91 ± 0.63 −1.53 ± 0.17 −2.52 ± 1.13 −0.99 ± 0.41 −3.02 ± 1.10 −0.63 ± 0.55
  1. Quantification by RT-qPCR assays A of 108 copies of the genome of viral RNA after monoazide treatment without photoactivation (A), after monoazide treatment without photoactivation followed by QIA-quick purification (B), after monoazide treatment with photoactivation followed by QIA-quick purification (C). Mean values ± SD (n=3).