Discrimination of infectious hepatitis A virus and rotavirus by combining dyes and surfactants with RT-qPCR

BMC Microbiology

Table 1 Binding of dyes to purified viral RNA

[Dye] μM	HAV		RV (Wa)		RV (SA11)
[Dye] μM	PMA	EMA	PMA	EMA	PMA	EMA
A
10	−0.09 ± 0.11	−0.12 ± 0.09	−0.87 ± 0.30	−0.52 ± 0.19	−1.47 ± 1.27	−0.41 ± 0.27
20	−1.59 ± 0.74	−0.21 ± 0.27	−1.87	−1.18 ± 0.46	−2.51 ± 0.69	−0.31 ± 0.31
50	< LOD	−0.99 ± 0.51	< LOD	< LOD	< LOD	−0.47 ± 0.15
100	< LOD	< LOD	< LOD	< LOD	< LOD	−0.44 ± 0.47
200	< LOD	< LOD	< LOD	< LOD	< LOD	−0.30 ± 0.41
B
0	−0.33 ± 0.10	−0.33 ± 0.10	−0.49 ± 0.51	−0.49 ± 0.51	−0.07 ± 0.26	−0.07 ± 0.26
10	−0.55 ± 0.13	−0.41 ± 0.26	−0.39 ± 0.11	−0.16 ± 0.06	−0.51 ± 0.16	−0.32 ± 0.32
20	−0.25 ± 0.27	0.37 ± 0.05	−0.27 ± 0.22	−0.37 ± 0.12	−0.68 ± 0.49	−0.28 ± 0.23
50	0.32 ± 0.26	0.43 ± 0.51	−0.34 ± 0.09	−0.23 ± 0.20	−1.60	−0.32 ± 0.23
100	−0.54 ± 0.01	0.03 ± 0.14	−0.38 ± 0.18	0.35 ± 0.24	< LOD	0.52 ± 0.23
200	−0.36 ± 0.13	0.35 ± 0.24	−0.30 ± 0.20	−0.47 ± 0.35	< LOD	−0.34 ± 0.16
C
0	−0.33 ± 0.10	−0.33 ± 0.10	−0.49 ± 0.51	−0.49 ± 0.51	−0.07 ± 0.26	−0.07 ± 0.26
10	−2.65 ± 0.51	−0.96 ± 0.27	−1.27 ± 0.12	−0.59 ± 0.24	−1.41 ± 0.51	−0.79 ± 0.50
20	−2.27 ± 0.46	−1.08 ± 0.48	−1.33 ± 0.13	−0.07 ± 0.50	−1.48 ± 0.55	−0.64 ± 0.66
50	−3.16 ± 0.77	−1.16 ± 0.21	−1.75 ± 0.11	−0.62 ± 0.38	−2.96 ± 1.38	−1.22 ± 0.67
100	−2.47 ± 0.37	−1.56 ± 0.33	−2.20 ± 0.50	−1.01 ± 0.11	−3.58 ± 0.65	−2.06 ± 1.63
200	−2.91 ± 0.63	−1.53 ± 0.17	−2.52 ± 1.13	−0.99 ± 0.41	−3.02 ± 1.10	−0.63 ± 0.55

Quantification by RT-qPCR assays A of 10⁸ copies of the genome of viral RNA after monoazide treatment without photoactivation (A), after monoazide treatment without photoactivation followed by QIA-quick purification (B), after monoazide treatment with photoactivation followed by QIA-quick purification (C). Mean values ± SD (n=3).

ISSN: 1471-2180