Table 2 Oligonucleotide primers used in this study
Primers | Sequence 5′-3′ | Target genes or regions | References |
|---|---|---|---|
27F | AGAGTTTGATCCTGGCTCAG | 16S rDNA | 46 |
1492R | GGTTACCTTGTTACGACTT | Â | Â |
attL-F | ATGACCAACCAGAACttyytnmayga | attL-int | This study* |
attL-R | ttywsnttyraCCCGGACCCGG | Â | Â |
Int-F | GACGCATTTCATCCAGG | int | This study |
Int-R | GCAACAGCGGGTAGACA | Â | Â |
attR-F | GGTTTAGCCACAGTTGTTC | attR | This study |
attR-R | CGTCAGGGTGCGAGAT | Â | Â |
PVP-F | GGCAATGGAAAAGAAGACGCGA | prfC of V. parahaemolyticus | 9 |
PVP-B | ATCAAACTCAGGACATGCACCC | Â | Â |
PVC-F | TCCTGCACCTTGCTCTGCTCT | prfC of V. cholerae | 9 |
PVC-B | ACCACGCTCTTTTTCCATTTCCAT | Â | Â |
setRpF | CGGCGGAGATGTTTTTGT | setR | 8 |
setRpR | GTGCGCCAATGCTCAGTT | Â | Â |
traC-F | TGACGCTGTTTTCACCAACG | traC | 8 |
traC-B | GGCACGACCTTTTTTCTCCC | Â | Â |
traI-F | GCAAGTCCTGATCCGCTATC | traI | 8 |
traI-R | CAGGGCATCTCATATGCGT | Â | Â |
LEFTF3 | GGTGCCATCTCCTCCAAAGTGC | rumBA (VRIII) | 39 |
RUMA | CGAGCAATCCCCACATCAAG | Â | Â |
HS1-F | GGTTCAGGCGTCATCTT | s043-traL | This study |
HS1-R | TCTCATCGGCACTCCA | Â | Â |
HS2-F | GTCGTTGCCAGCACTCA | traA-s054 | This study |
HS2-R | CGCCAGAATGATTGGAGAT | Â | Â |
HS3-F | GGTGTACTGGAAGACCGG | s073-traF | This study |
HS3-R | CAGGCAGCACTGAAAGG | Â | Â |
HS4-F | AGTGACCCAGGCATAGAC | traN-s063 | This study |
HS4-R | GAAGAGGAAACAGATAACCC | Â | Â |
E1 | TTGCGGGAGATTATGCTC | eex | 43 |
E2 | TGACCATCAATGAAGGTTG | Â | Â |
T1 | CATCTAGCGCCGTTGTTAATCAGGT | traG | 43 |
T2 | ATCGCGATACTCAGCACGTCGTGAA | Â | Â |
ctxA-F | CGGGCAGATTCTAGACCTCCTG | ctxA | 48 |
ctxA-R | CGATGATCTTGGAGCATTCCCAC | Â | Â |
L-TLH | AAAGCGGATTATGCAGAAGCACTG | tlh | 47 |
R-TLH GCT | ACTTTCTAGCATTTTCTCTGC | Â | Â |
tdh-1 | CCATCTGTCCCTTTTCCTGCC | tdh | 47 |
tdh-4c | CCACTACCACTCTCATATGC | Â | Â |
VPTRH-L | TTGGCTTCGATATTTTCAGTATCT | trh | 47 |
VPTRH-R | CATAACAAACATATGCCCATTTCCG | Â | Â |
P1 | TGCTGTCATCTGCATTCTCCTG | circular ICEs | 24 |
P2 | GCCAATTACGATTAACACGACGG | Â | Â |