Table 1 Full factorial CCD design matrix of four variables with the observed responses for CX produced by the bacterium D. natronolimnaea svgcc1.2736
Standard | D-glucose | Mg2+(MgSO4) | Mannose | Dose | CX(mg/1000 mL) | BDW(g/1000 mL) | ||
|---|---|---|---|---|---|---|---|---|
order | (g/L) | (ppm) | (g/L) | (Gy) | Â | Â | Â | Â |
| Â | Factor A | Factor B | Factor C | Factor D | Observed | Predicted | Observed | Predicted |
1 | 12.5 | 15 | 6.75 | 3.5 | 4.78±0.07 | 4.66 | 6.47±0.17 | 6.39 |
2 | 25 | 15 | 6.75 | 3.5 | 5.63±0.09 | 5.58 | 8.73±0.12 | 8.59 |
3 | 12.5 | 40 | 6.75 | 3.5 | 4.89±0.05 | 4.76 | 6.81±0.13 | 6.75 |
4 | 25 | 40 | 6.75 | 3.5 | 5.61±0.02 | 5.53 | 7.63±0.09 | 7.53 |
5 | 17.5 | 25 | 25 | 0.5 | 7.12±0.05 | 7.09 | 7.94±0.05 | 7.86 |
6 | 17.5 | 25 | 6.75 | 0.5 | 6.34±0.03 | 6.24 | 11.35±0.07 | 11.03 |
7 | 17.5 | 25 | 25 | 4.5 | 6.78±0.11 | 6.59 | 9.63±0.09 | 9.34 |
8 | 17.5 | 25 | 6.75 | 4.5 | 6.89±0.08 | 6.74 | 9.24±0.05 | 9.12 |
9 | 25 | 25 | 13.75 | 0.5 | 7.23±0.12 | 7.11 | 6.53±0.06 | 6.29 |
10 | 12.5 | 25 | 13.75 | 0.5 | 8.13±0.07 | 8.08 | 8.96±0.10 | 8.78 |
11 | 25 | 25 | 13.75 | 4.5 | 5.36±0.04 | 5.24 | 9.65±0.12 | 9.47 |
12 | 12.5 | 25 | 13.75 | 4.5 | 7.21±0.10 | 7.11 | 9.54±0.07 | 9.35 |
13 | 17.5 | 15 | 6.75 | 4.5 | 8.47±0.12 | 8.37 | 8.42±0.05 | 8.33 |
14 | 17.5 | 40 | 6.75 | 3.5 | 7.47±0.07 | 7.27 | 8.76±0.03 | 8.67 |
15 | 17.5 | 15 | 25 | 3.5 | 6.21±0.09 | 6.09 | 7.35±0.12 | 7.22 |
16 | 17.5 | 40 | 25 | 3.5 | 7.21±0.07 | 7.14 | 6.77±0.15 | 6.59 |
17 | 12.5 | 25 | 13.75 | 3.5 | 6.34±0.02 | 6.11 | 6.35±0.09 | 6.24 |
18 | 25 | 25 | 13.75 | 3.5 | 5.36±0.03 | 5.22 | 7.23±0.06 | 7.18 |
19 | 12.5 | 25 | 25 | 3.5 | 6.31±0.12 | 6.18 | 7.02±0.05 | 6.99 |
20 | 17.5 | 25 | 25 | 3.5 | 6.24±0.05 | 6.09 | 6.64±0.13 | 6.48 |
21 | 17.5 | 15 | 25 | 0.5 | 5.37±0.07 | 5.27 | 7.95±0.15 | 7.66 |
22 | 17.5 | 40 | 13.75 | 0.5 | 5.89±0.13 | 5.63 | 8.85±0.04 | 8.77 |
23 | 17.5 | 15 | 13.75 | 4.5 | 5.35±0.04 | 5.27 | 9.06±0.08 | 8.97 |
24 | 17.5 | 40 | 13.75 | 4.5 | 6.86±0.08 | 6.63 | 7.12±0.06 | 7.09 |
25 | 17.5 | 25 | 13.75 | 3.5 | 8.95±0.02 | 8.95 | 10.53±0.12 | 10.53 |
26 | 17.5 | 25 | 13.75 | 3.5 | 8.95±0.02 | 8.95 | 10.53±0.09 | 10.53 |
27 | 17.5 | 25 | 13.75 | 3.5 | 8.95±0.03 | 8.95 | 10.53±0.10 | 10.53 |
28 | 17.5 | 25 | 13.75 | 3.5 | 8.95±0.01 | 8.95 | 10.53±0.08 | 10.53 |
29 | 17.5 | 25 | 13.75 | 3.5 | 8.95±0.03 | 8.95 | 10.53±0.07 | 10.53 |
30 | 17.5 | 25 | 13.75 | 3.5 | 8.95±0.01 | 8.95 | 10.53±0.05 | 10.53 |