CaNIK1ΔHAMP expression led to growth inhibition that was dependent on His510 (A) and a functional HOG pathway (B). (A) Strains BWG1-7a, YES, NIK, ΔHa, ΔHaH510 and ΔH3H4 were streaked on SD-ura and SG-ura agar plates and incubated at 30°C for 4 days. Strain BWG1-7a was the parent strain which is auxotrophic for uracil. (B) Strains BY4741, ΔHbΔhog, ΔHbΔpbs2, ΔHbΔssk1, ΔHbH510 and ΔHb were streaked on SD-ura and SG-ura agar plates and incubated at 30°C for 4 days. BY4741 was the parent strain of the single gene deletion mutants, which is auxotrophic for uracil. SG-ura medium induced the expression of the transgenes via the galactose promoter.