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Figure 3 | BMC Microbiology

Figure 3

From: Deletion of the HAMP domains from the histidine kinase CaNik1p of Candida albicans or treatment with fungicides activates the MAP kinase Hog1p in S. cerevisiae transformants

Figure 3

Hog1p phosphorylation after fludioxonil treatment was dependent on the functionality of conserved domains of CaNik1p. The phosphorylation of Hog1p (upper panel, Hog1-P) was detected by Western blot after treatment of the strains YES, NIK, N627, D924 and H510 with fludioxonil (10 μg/ml) and sorbitol (1 M), respectively, for 15 min. The presence of Hog1p in all strains was proven (lower panel, Hog1). Hog1p appeared at approximately 50 kDa.

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