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Table 3 Strains and plasmids used in this study

From: MamX encoded by the mamXY operon is involved in control of magnetosome maturation in Magnetospirillum gryphiswaldense MSR-1

Strains and plasmids

Description

Source or reference

Strains

  

M. gryphiswaldense MSR-1

wild-type, Nxr

DSM6361

M. gryphiswaldense MSR-1 ΔmamX

mamX deficient mutant, Nxr Gmr

present study

M. gryphiswaldense MSR-1 CmamX

complementation of ΔmamX, NxrGmrTcr

present study

E. coli DH5α

endA1 hsdR17 (r- m+) supE44 thi-1 recA1 gyrA (NalR) recA1 Δ (lacZYA-argF)U169 deoR [Ø80ΔdlacZ ΔM15]

[35]

E. coli S17-1

thi endA recA hsdR with RP4-2-Tc::Mu-Km::Tn7 integrated in chromosome, Smr

[36]

Plasmids

  

pUCGm

pUC1918 carrying the aacC1 gene, Gmr

[37]

pSUP202

suicide vector for M. gryphiswaldense MSR-1, CmrTcr Ampr

[38]

pSUPpX2

pSUP202 derivative for mamX deletion, GmrCmrAmpr

present study

pRK415

Cloning vector, pRK290 derivative, Tcr

[39]

pRK415X

pRK415 derivative for mamX expression, Tcr

present study