Table 2 Primers used for characterization and real-time PCR
Gene | Forward primer (5’-3’) | Reverse primer (5’-3’) | Reference |
|---|---|---|---|
16S | CGGGGAGGAAGGTGTTGTG | GAGCCCGGGGATTTCACATC | [38] |
hilA | CGCTGGCAGAATGCTACCTC | AGCCCCAGTAATCCTAAAGCTTG | [39] |
prgH | GCTCTTTCTTGCTCATCGT | ATCTCTATCTGGCTGGATACCT | This study |
invF | ATGTGAAGGCGATGAGTAAC | GCTGCTGAATAGTGTAGAAGG | This study |
tetA | GCTACATCCTGCTTGCCTTC | CATAGATCGCCGTGAAGAGG | [13] |
tetB | GCTTTCAGGGATCACAGGAG | CCAAGACCCGCTAATGAAAA | [13] |
tetC | GCATAAACCAGCCATTGAG | GGTAAACGCCATTGTCAG | This study |
tetD | GATGTGGCGAATAAAGCG | CCAGTGTGACCCCTGTTAC | This study |
tetG | CCTTGCAGGCAATGCTCTCAAACA | AGATTGGTGAGGCTCGTTAGCGTT | [40] |
thdF-S001 | GGGACGGATTTTCTCCAG | CGAGTTAGGGTTACGCTTG | This study |
S013 | CCACAATTTGGCTGTGATGGCTCA | TGGTCGTGTTATTAGCGGCCAGAT | [40] |
S044 | TGGACGCTCGAAGAGGTAGAG | AAGGATGGCCGTCGTCACT | [40] |