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Table 1 Up- or down-regulated Brucella suis proteins under nutrient starvation conditions

From: Quantitative analysis of the Brucella suis proteome reveals metabolic adaptation to long-term nutrient starvation

Spot IDa

ORFb

Protein functionc

Theoret.Mr/pId

Fold changee

t-Testf

 

Adaptation to atypical conditions

 

2146

BR2149

Dps family protein (DNA-binding proteins from starved cells)

18.2/5.3

2.63

0.00019

429

BR0685

organic solvent tolerance, putative

88.7/5.4

1.53

0.024

2122

BR2149

Dps family protein

18.2/5.3

1.52

0.006

438

BR0685

organic solvent tolerance, putative

88.7/5.3

1.49

0.0004

 

Stress proteins/chaperones, protein folding

 

1624

BR0171

heat shock protein GrpE

25.2/4.7

−1.42

0.039

662

BR2125

chaperone protein DnaK

68.2/4.9

1.65

0.0056

 

Cell envelope

 

1653

BRA0423

31 kDa outer-membrane immunogenic protein (“Omp31-2”)

23.2/5.2

1.45

0.00034

1874

BRA0423

31 kDa outer-membrane immunogenic protein (“Omp31-2”)

23.2/5.2

1.34

0.026

 

Transport and binding proteins

 

1415

BR0639

porin Omp2a (omp2b)

40.5/4.6

1.41

0.03

1410

BR0639

porin Omp2a (omp2b)

40.5/4.6

1.4

0.028

2176

BRA0565

bacterioferritin

18.7/4.6

1.38

0.00065

1229

BRA0655

glycerol-3-phosphate ABC transporter, periplasmic

47.2/5.4

1.33

0.0043

 

Energy metabolism

 
 

ATP-proton motive force interconversion

 

1019

BR1800

ATP synthase F1, gamma subunit

32.0/7.8

1.6

0.021

 

Electron transport

 

1435

BRA0893

thioredoxin

34.7/4.8

−1.34

0.0045

 

Glycolysis/TCA cycle

 

1145

BR1132

enolase

45.4/5.0

1.43

0.0021

 

Amino acid metabolism

 
 

Biosynthesis

 

1915

BRA0883

3-isopropylmalate dehydratase, small subunit

22.5/5.0

−1.55

0.0013

221

BR1488

carbamoyl-phosphate synthase, large subunit

126.9/5.0

−1.34

0.0098

 

Degradation

 

278

BRA0725

glycine cleavage system P protein

99.9/5.8

1.51

0.00044

 

Transport

 

1219

BRA1193

amino acid ABC transporter

44.2/5.6

1.38

0,000015

1293

BRA0953

amino acid ABC transporter, periplasmic amino acid-binding protein, putative

43.3/5.3

1.36

0.0019

1549

BR0741

amino acid ABC transporter, periplasmic amino acid binding protein

37.2/5.3

1.31

0.00014

 

Protein metabolism

 
 

Biosynthesis

 

1783

BR0455

ribosomal protein S6

17.1/8.0

1.69

0.0069

1980

BR0452

ribosomal protein L9

21.0/4.8

1.59

0.00041

 

Secretion

 

313

BR1945

preprotein translocase, SecA subunit

103.0/5.1

−1.34

0.005

 

DNA/RNA metabolism

 
 

Biosynthesis

 

221

BR1488

carbamoyl-phosphate synthase, large subunit

126.9/5.0

−1.34

0.0098

454

BR0837

phosphoribosylformylglycinamidine synthase II

80.0/4.8

−1.31

0.01

456

BR0837

phosphoribosylformylglycinamidine synthase II

80.0/4.8

−1.31

0.015

 

Degradation

 

689

BR2169

polyribonucleotide nucleotidyltransferase

77.7/5.0

1.55

0.0029

 

Fatty acid metabolism

 
 

Degradation

 

1881

BR1510

long-chain acyl-CoA thioester hydrolase, putative

14.25/6.6

1.67

*

 

Sugar metabolism

 
 

Transport

 

1642

BR0544

ribose ABC transporter, periplasmic D-ribose-binding

34.6/4.8

1.46

*

 

Regulation

 

1743

BR0569

transcriptional regulator, Ros/MucR family

16.10/7.8

1.73

0.021

1843

BR2159

transcriptional regulator, Cro/Cl family

15.1/9.0

1.6

*

1813

BR1502

leucine-responsive regulatory protein

17.8/6.7

1.5

0.049

 

Oxidoreduction

 

1975

BRA0708

alkyl hydroperoxide reductase C

20.6/5.0

−1.39

0.005

 

Cofactor biosynthesis

 

826

BRA0491

8-amino-7-oxononanoate synthase

40.6/7.3

1.52

0.033

 

Unknown function

 

2190

BRA0336

conserved hypothetical protein

18.4/5.0

−1.42

0. 022

  1. aThe indicated number is an arbitrary designation of the annotated spots on the 2D proteome maps [see Additional files 1 and 2].
  2. bOpen reading frame number attributed by Paulsen et al. [20].
  3. cAs annotated by Paulsen et al. [20].
  4. dCalculated from the amino acid sequence of the translated open reading frame.
  5. eIncrease or decrease of protein concentrations after normalization of protein spot intensities from 2D-DIGE gels of B. suis recovered from a 6-weeks-starvation condition as compared to normalized protein spot intensities of corresponding spots from early stationary phase control of B. suis in TS broth.
  6. fStatistical significance of the ratio described ine.
  7. *indicates lack of statistical validation of the ratio described ine, but increase in protein concentration was observed in each of the three independent experiments.
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BMC Microbiology

ISSN: 1471-2180

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