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Table 4 Flow cytometry analysis of ΔΨm and ROS production in T. cruzi epimastigotes

From: Trypanosoma cruzi mitochondrial swelling and membrane potential collapse as primary evidence of the mode of action of naphthoquinone analogues

Cpd   TMRE DHE
   % cells+ IVa % cells+
-   97.9 ± 1.8b 0.00 3.9 ± 1.8
- + 10 μM FCCP 3.4 ± 1.5 −0.70* - c
- + 22 μM AA - - 71.8 ± 14.5
NQ1 0.1 μM 98.6 ± 1.7 0.04 6.4 ± 3.3
  0.2 μM 98.3 ± 1.5 −0.07 4.7 ± 2.2
  0.3 μM 96.1 ± 4.1 −0.22* 4.8 ± 2.7
NQ8 0.2 μM 97.4 ± 3.1 −0.18* 2.1 ± 0.8
  0.4 μM 93.4 ± 3.1 −0.33* 2.9 ± 1.5
  0.8 μM 76.7 ± 14.4 −0.53* 26.1* ± 9.9
NQ9 0.6 μM 98.5 ± 0.9 0.09 5.9 ± 2.0
  1.3 μM 96.0 ± 5.1 0.04 5.0 ± 2.7
  2.6 μM 92.2 ± 7.8 −0.27* 7.5 ± 4.7
NQ12 0.1 μM 98.2 ± 1.9 0.08 6.3 ± 2.7
  0.2 μM 97.1 ± 3.8 0.05 5.4 ± 3.6
  0.5 μM 97.7 ± 1.3 −0.22* 7.2 ± 3.8
  1. aIV = (MT – MC)/MC, where MT corresponds to the marker median fluorescence for treated parasites, and MC corresponds to that of control parasites. Negative IV values correspond to depolarization of the mitochondrial membrane.
  2. bMean ± standard deviation of 4 independent experiments.
  3. cNot determined.
  4. Asterisks indicate significant differences in relation to the control group (* p ≤ 0.002).