BMC Microbiology

Table 4 Flow cytometry analysis of ΔΨm and ROS production in T. cruzi epimastigotes

From: Trypanosoma cruzi mitochondrial swelling and membrane potential collapse as primary evidence of the mode of action of naphthoquinone analogues

Cpd		TMRE		DHE
		% cells+	IV^a	% cells+
-		97.9 ± 1.8^b	0.00	3.9 ± 1.8
-	+ 10 μM FCCP	3.4 ± 1.5	−0.70*	- ^c
-	+ 22 μM AA	-	-	71.8 ± 14.5
NQ1	0.1 μM	98.6 ± 1.7	0.04	6.4 ± 3.3
	0.2 μM	98.3 ± 1.5	−0.07	4.7 ± 2.2
	0.3 μM	96.1 ± 4.1	−0.22*	4.8 ± 2.7
NQ8	0.2 μM	97.4 ± 3.1	−0.18*	2.1 ± 0.8
	0.4 μM	93.4 ± 3.1	−0.33*	2.9 ± 1.5
	0.8 μM	76.7 ± 14.4	−0.53*	26.1* ± 9.9
NQ9	0.6 μM	98.5 ± 0.9	0.09	5.9 ± 2.0
	1.3 μM	96.0 ± 5.1	0.04	5.0 ± 2.7
	2.6 μM	92.2 ± 7.8	−0.27*	7.5 ± 4.7
NQ12	0.1 μM	98.2 ± 1.9	0.08	6.3 ± 2.7
	0.2 μM	97.1 ± 3.8	0.05	5.4 ± 3.6
	0.5 μM	97.7 ± 1.3	−0.22*	7.2 ± 3.8

^aIV = (MT – MC)/MC, where MT corresponds to the marker median fluorescence for treated parasites, and MC corresponds to that of control parasites. Negative IV values correspond to depolarization of the mitochondrial membrane.
^bMean ± standard deviation of 4 independent experiments.
^cNot determined.
Asterisks indicate significant differences in relation to the control group (* p ≤ 0.002).

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ISSN: 1471-2180

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